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J Vet Sci. 2004 Dec; 5(4): 391-395 |
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Isolation of cholesterol-lowering lactic acid bacteria from human intestine for probiotic use
Hyeong-Jun Lim1, So-Young Kim2, Wan-Kyu Lee1,*. |
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1College of Veterinary Medicine and Research Institute of Veterinary Medicine, Chungbuk National University, Cheongju 361-763, Korea. wklee@cbu.ac.kr
2Department of Food Science and Technology, and Research Center for Bioresource and Health, Chungbuk National University, Cheongju 361-763, Korea. |
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Cholesterol-lowering effect of lactic acid bacteria (LAB: Streptococcus, Lactobacillus and Bifidobacterium) is well-known. Thus, we investigated LAB isolated from human intestine on the cholesterol-lowering effect in vitro. Seven Streptococcus (61.1%), 11 Lactobacillus (71.8%) and 7 Bifidobacterium (27.9%) were isolated as acid (pH 2.5 and 3.0) and bile (0.3% oxgall) tolerant strains. Streptococcus HJS-1, Lactobacillus HJL-37 and Bifidobacterium HJB-4 were finally selected as probiotic strains to use through the bile salt hydrolase (BSH) activity assay by using MRS media added taurodeoxycholic acid (TDCA) and the cholesterol-lowering test by using soluble cholesterol containing MRS broth. These studies suggested that the isolated LAB had an excellent hypocholesterolemic effect.
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