Ofloxacin was administered to six male goats intravenously (5 mg/kg) to determine its kinetic behavior, tissue residue, in vitro plasma protein binding and to compute a rational dosage regimen. The concentration of ofloxacin in plasma and tissue samples collected at prescheduled time were estimated by using HPLC. The pharmacokinetic parameters were determined by non-compartmental model and plasma protein binding was estimated by equilibrium dialysis technique. The therapeutic concentration (≥0.5 µg/ml) was maintained up to 36 h and the initial concentration at 2.5min (14.76 ± 0.47 µg/ml) declined to 0.05 ± 0.03 µg/ml at 96 h with a secondary peak (0.64 ± 0.15 µg/ml) at 24 h. The mean AUC, AUMC, t1/2, MRT, Cl and Vd were calculated to be 58.94 ± 19.43 µg h/ml, 1539.57 ± 724.69 µg h2/ml, 15.58 ± 1.87 h, 22.46 ± 2.71 h, 135.60 ± 31.12 ml/h/kg and 2.85 ± 0.74 L/kg respectively. Significantly high concentration of drug was detected in different tissues after 24 h of intravenous dosing of 5mg/kg, at 24 h interval for 5 days. The in vitro plasma protein binding of ofloxacin was found to be 15.28 ± 0.94%. Based on these kinetic parameters, a loading dose of 5mg/kg followed by the maintenance dose of 3mg/kg at 24 h dosing interval by intravenous route is recommended.