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J Vet Sci. 2005 Jun;6(2):157-160 |
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Exploration of immunoblot profiles of Neospora caninum probed with different bovine immunoglobulin classes
Yong-Seung Shin1, Eung-Goo Lee2, Tae-Sung Jung1,* |
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1Institue of Animal Medicine, College of Veterinary Medicine, Gyeongsang National University, Jinju 660-701, Korea. 2Department of Molecular Parasitology, School of Medicine, Sungkyunkwan University, Suwon 440-746, Korea. |
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The present study was attempted to compare the
Neospora caninum (N. caninum) antigenic bands recognized
by different bovine immunoglobulin classes. A total 10, 5,
2, and 6 antigenic bands were exhibited on immunoblot
profiles against bovine IgM, IgE, IgA, and IgG, respectively.
A 46 kDa band was probed as a common antigenic band
except IgA; 69 kDa band was bovine IgM and IgE; 33, 37,
55, and 79 kDa bands were bovine IgM and IgG; 72 kDa
band was found IgM and IgA profiles. Based on the
analysis, it appeared that different immunoglobulin
classes recognizing different antigenic molecules were
cooperating to cope with neosporosis. |
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