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J Vet Sci. 2006 Jun;7(2):181-187 |
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Random amplified polymorphic DNA (RAPD) analysis of Mycobacterium tuberculosis strains in India
J. P. N. Singh1, Rishendra Verma1,*, P. Chaudhuri2 |
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1Division of Bacteriology and Mycology, Indian Veterinary Research Institute, Izatnagar-243122, U.P., India. rishendra_verma@yahoo.com
2Division Veterinary Biotechnology, Indian Veterinary Research Institute, Izatnagar-243122, U.P., India |
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The usefulness of random amplification of polymorphic
DNA (RAPD) analysis for typing Indian strains of M.
tuberculosis was investigated. M. tuberculosis H37Rv, M.
tuberculosis DT and 42 clinical isolates of M. tuberculosis
were subjected to RAPD-PCR using 7 random decamer
primers. All 7 primers were found to be differentiated and
produced specific RAPD profiles. The polymorphic amplicons
served as RAPD markers for M. tuberculosis. The
dendrograms, obtained by different primers, showed the
discriminatory ability of the primers. RAPD analysis
provided a rapid and easy means of identifying polymorphism
in M. tuberculosis isolates, and it was found to be a
valuable alternative epidemiological tool. In addition, the
results of the present study showed heterogeneity in the
M. tuberculosis strains in the population studied.
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