|
        |
     |
| |
J Vet Sci. 2006 Mar;7(1):59-61 |
 |
| |
Some observations
on the adaptation of Eimeria tenella (local isolates)
sporozoites on chicken embryos through chorioallantoic membrane
M.Abdul Hafeez, Masood Akhtar*, M.Mazhar
Ayaz |
| |
Immunoparasitology
Laboratory, Department of Veterinary Parasitology, University
of Agriculture, Faisalabad-38040, Pakistan. drakhtar@brain.net.pk |
| |
Eimeria (E.)tenella (local isolate)sporzoites
were
adapted on the chorioallantoic membrane (CAM)of 10-12
days chicken embryos and completed its life cycle in 6~7
days at 39oC and 70 per cent humidity.Only 23 embryos
(4.6%)were found dead from 1~4 day post inoculation of sporozoites with mild lesions on CAM with no gametocytes
but few sporozoites in chorioallantoic fluid (CAF).On 5~7
day post inoculation, 432 embryos (86.4%)were found
dead with severe haemorrhages on CAM and CAF contained
uncountable number of gametocytes.After seven days
post inoculation, 45 embryos (9%)were found to be alive.
Some oocysts were also detected in the CAF on 6~7 days
post inoculation.In the histological sections of the CAM,
there were abundant small dark colored rounded bodies of
gametes;distributed extensively in tissues of CAM on 5~7
days post inoculation of sporozoites.In some cases, cluster
of small mature and immature relatively large bodies were
seen in increasing numbers on 5~6 days post inoculation.
|
|
| |
|
