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J Vet Sci. 2006 Mar;7(1):63-37 |
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Parentage testing
of Thoroughbred horse in Korea using microsatellite DNA typing
Sun-young Lee1 , Gil-jae Cho 2, * |
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1 Laboratory
of Equine Genetics, Korea Racing Association, Gwacheon 427-711, Korea. 2 College of Veterinary Medicine, Kyungpook National
University, Daegu 702-701, Korea |
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The present study was to construct a parentage
testing system for Thoroughbred (TB)horse.A total number
of 1, 285 TB horse samples including 962 foals for parentage
testing, 9 sires and 314 dams for individual identification
were genotyped.Genomic DNA was extracted from 5 hair roots
and genotyped by using 14 microsatellite markers (AHT4, AHT5, ASB2, ASB17, ASB23, CA425, HMS1,
HMS3, HMS6, HMS7, HTG4, HTG10, LEX3 and VHL20). This method consisted
of multiplexing PCR procedure and showed reasonable amplification
of all PCR products. Genotypes were determined by genetic
analyzer. The number of alleles per locus varied from 3
to 9 with a mean value of 6.36 in TB horse.The expected
heterozygosity was ranged from 0.548 to 0.831 (mean 0.699), and
the total exclusion probability of 14 microstellite loci
was 0.9998. Of the 14 markers, ASB2, ASB17, ASB23, HMS7
and HTG10 loci have relatively high PIC value (>0.7).Of
the 962 foals, 960 foals were qualified by compatibility
according to the Mendelism.These results suggest that the
DNA typing method has high potential for parentage verification
and individual identification of TB horses.
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