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J Vet Sci. 2007 Sep;8(3):209-212 |
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The expression and cellular localization of phospholipase D isozymes in the developing mouse testis
Seungjoon Kim1,2,ΆΣ, Heechul Kim1,2,ΆΣ, Yongduk Lee1, Jin Won Hyun2,3, Young Ho Lee4, Min Kyoung Shin5, Do Sik Min5,*, Taekyun Shin1,2,* |
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1Department of Veterinary Medicine, College of Applied Life Sciences, 2Applied Radiological Science Research Institute, and 3Department of Biochemistry, College of Medicine, Cheju National University, Jeju 690-756, Korea
4Department of Anatomy, College of Medicine, Chungnam National University, Daejeon 301-131, Korea
5Department of Molecular Biology, College of Natural Science, Pusan National University, Busan 609-735, Korea
* shint@cheju.ac.kr, minds@pusan.ac.kr |
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To examine the involvement of phospholipase D (PLD)
isozymes in postnatal testis development, the expression of
PLD1 and PLD2 was examined in the mouse testis at
postnatal weeks 1, 2, 4, and 8 using Western blot analysis
and immunohistochemistry. The expression of both PLD1
and PLD2 increased gradually with development from
postnatal week 1 to 8. Immunohistochemically, PLD
immunoreactivity was detected in some germ cells in the
testis and interstitial Leydig cells at postnatal week 1.
PLD was mainly detected in the spermatocytes and
residual bodies of spermatids in the testis after 8 weeks
after birth. The intense immunostaining of PLD in Leydig
cells remained unchanged by postnatal week 8. These
findings suggest that PLD isozymes are involved in the
spermatogenesis of the mouse testis.
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