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J Vet Sci. 2007 Sep;8(3):303-305 |
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Survival of avirulent thermostable Newcastle disease virus (strain I-2) in raw, baked, oiled, and cooked white rice at ambient temperatures
Philemon Nyangi Wambura1,*, Joanne Meers2, Peter Spradbrow2 |
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1Department of Veterinary Microbiology and Parasitology, Faculty of Veterinary Medicine, Sokoine University of Agriculture, P. O. Box 3019, Morogoro, Tanzania
2School of Veterinary Science, University of Queensland, Brisbane, QLD 4072, Australia
* phil_wambura@yahoo.com, pwambura@suanet.ac.tz |
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Raw white rice has not been considered a good carrier
for oral vaccination, probably because of its antiviral
activity. Methods are required to overcome antiviral
activity in raw white rice. This study was carried out to
determine the effects of various treatments of raw white
rice on the survival of strain I-2 of Newcastle disease
virus. These included cooking and baking the rice or
mixing the rice with vegetable oil prior to coating with
vaccine virus. The vaccine-coated rice was then stored for
30 min and 24 h, followed by quantitative recovery of the
virus. Thirty min after mixing, uncooked, cooked, and
baked rice, and rice mixed with vegetable oil showed titers
of 106.2, 107.2, 106.6, and 107.0 EID50/0.1 ml, respectively.
After storage for 24 h at 22-25oC, the titers dropped to
105.0, 106.5, 105.0, and 106.0 EID50/0.1 ml for uncooked,
cooked, baked, and oiled rice, respectively.
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