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J Vet Sci. 2007 Dec;8(4):409-414 |
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The determination of dark adaptation time using electroretinography in conscious Miniature Schnauzer dogs
Hyung-Ah Yu¢Ó, Man-Bok Jeong¢Ó, Shin-Ae Park, Won-Tae Kim, Se-Eun Kim, Je-Min Chae, Na-Young Yi,
Kang-Moon Seo* |
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Department of Veterinary Surgery and Ophthalmology, College of Veterinary Medicine and BK21 Program for Veterinary Science, Seoul National University, Seoul 151-742, Korea
* kmseo@snu.ac.kr |
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The optimal dark adaptation time of electroretinograms
(ERG's) performed on conscious dogs were determined
using a commercially available ERG unit with a contact
lens electrode and a built-in light source (LED-electrode).
The ERG recordings were performed on nine healthy
Miniature Schnauzer dogs. The bilateral ERG's at seven
different dark adaptation times at an intensity of 2.5
cd¤ýs/m©÷ was performed. Signal averaging (4 flashes of
light stimuli) was adopted to reduce electrophysiologic
noise. As the dark adaptation time increased, a significant
increase in the mean a-wave amplitudes was observed in
comparison to base-line levels up to 10 min (p £¼ 0.05).
Thereafter, no significant differences in amplitude occured
over the dark adaptation time. Moreover, at this
time the mean amplitude was 60.30 ¡¾ 18.47 ¥ìV. However,
no significant changes were observed for the implicit times
of the a-wave. The implicit times and amplitude of the
b-wave increased significantly up to 20 min of dark adaptation
(p £¼ 0.05). Beyond this time, the mean b-wave amplitudes
was 132.92 ¡¾ 17.79 ¥ìV. The results of the present
study demonstrate that, the optimal dark adaptation time
when performing ERG's, should be at least 20 min in conscious
Miniature Schnauzer dogs.
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