J Vet Sci. 2007 Dec;8(4):409-414   
 

The determination of dark adaptation time using electroretinography in conscious Miniature Schnauzer dogs

Hyung-Ah Yu¢Ó, Man-Bok Jeong¢Ó, Shin-Ae Park, Won-Tae Kim, Se-Eun Kim, Je-Min Chae, Na-Young Yi, Kang-Moon Seo*

 

Department of Veterinary Surgery and Ophthalmology, College of Veterinary Medicine and BK21 Program for Veterinary Science, Seoul National University, Seoul 151-742, Korea
* kmseo@snu.ac.kr

 

The optimal dark adaptation time of electroretinograms (ERG's) performed on conscious dogs were determined using a commercially available ERG unit with a contact lens electrode and a built-in light source (LED-electrode). The ERG recordings were performed on nine healthy Miniature Schnauzer dogs. The bilateral ERG's at seven different dark adaptation times at an intensity of 2.5 cd¤ýs/m©÷ was performed. Signal averaging (4 flashes of light stimuli) was adopted to reduce electrophysiologic noise. As the dark adaptation time increased, a significant increase in the mean a-wave amplitudes was observed in comparison to base-line levels up to 10 min (p £¼ 0.05). Thereafter, no significant differences in amplitude occured over the dark adaptation time. Moreover, at this time the mean amplitude was 60.30 ¡¾ 18.47 ¥ìV. However, no significant changes were observed for the implicit times of the a-wave. The implicit times and amplitude of the b-wave increased significantly up to 20 min of dark adaptation (p £¼ 0.05). Beyond this time, the mean b-wave amplitudes was 132.92 ¡¾ 17.79 ¥ìV. The results of the present study demonstrate that, the optimal dark adaptation time when performing ERG's, should be at least 20 min in conscious Miniature Schnauzer dogs.