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- J Vet Sci. 2009 Jun;10(2):115-120
DOI: 10.4142/jvs.2009.10.2.115 |
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Genetic variability of the prion protein gene (PRNP) in wild ruminants
from Italy and Scotland
Simone Peletto1,*, Matteo Perucchini2, Cristina Acin3, Mark P. Dalgleish4, Hugh W. Reid4, Roberto Rasero5, Paola Sacchi5, Paula Stewart2, Maria Caramelli1, Ezio Ferroglio5, Elena Bozzetta1, Daniela Meloni1, Riccardo Orusa1, Serena Robetto1, Silvia Gennero1, Wilfred Goldmann2, Pier Luigi Acutis1 |
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1Istituto Zooprofilattico Sperimentale del Piemonte, Liguria e Valle dĄŻAosta, 10154 Turin, Italy
2Neuropathogenesis Division, The Roslin Institute and Royal (Dick) School of Veterinary Studies, University of Edinburgh,
Roslin, Midlothian, EH25 9PS, UK
3Department of Animal Pathology, Faculty of Veterinary Medicine, University of Zaragoza, 50013 Zaragoza, Spain
4Moredun Research Institute, Pentlands Science Park, Bush Loan, Penicuik, Midlothian EH26 0PZ, UK
5Department of Animal Productions, Epidemiology and Ecology, Faculty of Veterinary Medicine, University of Turin, 10095
Grugliasco, Italy
* simone.peletto@izsto.it |
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The genetics of the prion protein gene (PRNP) play a
crucial role in determining the relative susceptibility to
transmissible spongiform encephalopathies (TSEs) in
several mammalian species. To determine the PRNP gene
variability in European red deer (Cervus elaphus), roe deer
(Capreolus capreolus) and chamois (Rupicapra rupicapra), the
PRNP open reading frame from 715 samples was analysed to
reveal a total of ten single nucleotide polymorphisms (SNPs).
In red deer, SNPs were found in codons 15, 21, 59, 78, 79, 98,
136, 168 and 226. These polymorphisms give rise to 12
haplotypes, and one of which is identical to the PRNP of
American wapiti (Rocky Mountain elk, Cervus elaphus
nelsoni). One silent mutation at codon 119 was detected in
chamois and no SNPs were found in roe deer. This
analysis confirmed that European wild ruminants have a
PRNP genetic background that is compatible with TSE
susceptibility, including chronic wasting disease.
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