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- J Vet Sci. 2009 Jun;10(3):233-238 DOI: 10.4142/jvs.2009.10.3.233 |
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The normal electroretinogram in adult healthy Shih Tzu dogs using the
HMsERG
June-sub Lee1, Kyung-hee Kim1, Ha-young Jang1, Bora Lee1, Joon Young Kim2, Soon-wuk Jeong1,* |
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1Department of Veterinary Surgery, College of Veterinary Medicine, Konkuk University, Seoul 143-701, Korea
2Department of Veterinary Medicine, Madingley Road, Cambridge CB3 0ES, United Kingdom
* swjeong@konkuk.ac.kr |
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Electroretinography (ERG) is a reliable diagnostic tool
for the diagnosis of retinal disease. It measures electric
potentials occurring in the retina in response to light
stimulation. In this study, we examined the normal
electroretinogram using the Handheld Multispecies ERG
(HMsERG) in Shih Tzu dogs. ERG recordings were
performed in twelve eyes of six healthy Shih Tzu dogs.
Dogs were anesthetized with a combination of medetomidine
and ketamine. Proparacaine eye drops were also applied
as a topical anesthetic. Tropicamide eye drops were
applied for mydriasis. After 20 min of dark adaptation, we
recorded the amplitudes and implicit times of the b-waves
of the rod, standard rod and cone (Std R&C), highintensity
rod and cone (Hi-int R&C), and cone systems,
and responses of the cones and inner retina by flicker light
stimulation (cone flicker). Results showed that mean the
amplitudes of a-waves of Std R&C, Hi-int R&C, and the
cone responses were 141.25 レV, 173.00 レV, and 12.92 レV,
respectively. The b-waves of the rod responses ranged
from 141.58 to 155.25 レV; the Std R&C was 314.75 レV,
the Hi-int R&C was 329.42 レV, the cones were 37.75 レV,
and the flicker responses were 64.08 レV. The b/a ratios for
the Std R&C, Hi-int R&C, and the cone response were
2.29, 1.94, and 3.71, respectively. Mean implicit time of the
a-wave of the Std R&C was 15.12 ms, of Hi-int R&C was
13.42 ms, and of the cone response was 7.22 ms. The
b-wave of the rod responses ranged from 68.12 to 72.68
ms, of Std R&C were 37.28 ms, of Hi-int R&C were 41.90,
of the cone responses were 38.12 ms, and of the cone
flicker responses were 22.80 ms. We believe that these
parameters can be used as reference ^normal ̄ ERGs
ranges for Shih Tzu dogs using the HMsERG under
medetomidine and ketamine anesthesia.
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