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- J Vet Sci. 2009 Jun;10(3):261-263
DOI: 10.4142/jvs.2009.10.3.261 |
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Isolation and identification of a canine coronavirus strain from giant
pandas (Ailuropoda melanoleuca)
Feng-Shan Gao1, Gui-Xue Hu1,2,*, Xian-zhu Xia3, Yu-Wei Gao3, Ya-Duo Bai2, Xiao-Huan Zou3 |
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1Department of Biochemistry and Molecular Biology, College of Bioengineering, Dalian University, Dalian, Liaoning 116622, China
2Department of Microbiology and Immunology, College of Animal Science of Jilin Agricultural University, Changchun, Jilin
130118, China
3Institute of Military Veterinary Science, Academy of Military Medical Science, Changchun, Jilin 130062, China
* huguixue90110@126.com |
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Two giant pandas (Ailuropoda melanoleuca) died of
unknown causes in a Chinese zoo. The clinical disease
profile suggested that the pandas may have suffered a
viral infection. Therefore, a series of detection including
virus isolation, electron microscopy, cytobiological assay,
serum neutralization and RT-PCR were used to identify
the virus. It was determined that the isolated virus was a
canine coronavirus (CCV), on the basis of coronavirus,
neutralization by canine anti-CCV serum, and 84.3% to
100% amino acid sequence similarity with CCV. The
results suggest that the affected pandas had been infected
with CCV.
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