Effect of donor age on the proliferation and multipotency of canine adipose-derived mesenchymal stem cells
Jienny Lee, Keum Sil Lee, Chan-Lan Kim, Jeong Su Byeon, Na-Yeon Gu, In-Soo Cho, Sang-Ho Cha*
Viral Disease Division, Animal and Plant Quarantine Agency, Gimcheon 39660, Korea
Correspondence to: Tel: +82-54-912-0809; Fax: +82-54-912-0812; E-mail: virusmania@korea.kr
The first two authors contributed equally to this work.
Received: August 24, 2015; Revised: April 19, 2016; Accepted: June 8, 2016; Published online: July 25, 2016.
Abstract
Adipose-derived mesenchymal stem cells (AD-MSCs) have demonstrated the feasibility of the usage in clinical application, due to ease of isolation and abundance in tissue. We isolated MSCs from adipose tissues of young and old dogs, which were subjected to sequential sub-passages passage 1 (P1) to P7. The canine AD-MSCs (cAD-MSCs) were examined for proliferation kinetics and expression of molecules associated with self-renewal, expression of MSC-specific surface markers and differentiation capabilities at P3. The cumulative population doubling level was significantly higher in MSCs of young donors than in those of old donors. In addition, expression of CD73, CD80, Oct3/4, Nanog, and cell survival genes in young donors was significantly higher when compared with that of old donors. Also, differentiation into mesodermal cell lineages was considerably decreased in old donors than in young donors. The present study suggested that donor age should be considered in developing cell-based therapies for clinical application of cAD-MSCs.
Keywords: adipose mesenchymal stem cells, age, canine, differentiation, multipotency


© 2016 The Korean Society of Veterinary Science.