Immunogenicity of recombinant Lactobacillus plantarum NC8 expressing goose parvovirus VP2 gene in BALB/c mice
Yu-Ying Liu1, Wen-Tao Yang1, Shao-Hua Shi1, Ya-Jie Li1, Liang Zhao1, Chun-Wei Shi1,Fang-Yu Zhou1, Yan-Long Jiang1,Jing-Tao Hu1,Wei Gu1,2, Gui-Lian Yang1,*, Chun-Feng Wang1,*
1College of Animal Science and Technology, Jilin Provincial Engineering Research Center of Animal Probiotics, Jilin Agricultural University, Changchun 130118, China
2Shandong Baolai-leelai bioengineering Co.,Ltd, Taian 271000, China
Correspondence to: Tel/Fax: +86-43184533425; E-mail: (GL Yang), (CF Wang).
Received: September 25, 2015; Revised: April 11, 2016; Accepted: June 8, 2016; Published online: July 25, 2016.
Goose parvovirus (GPV) continues to be a threat that has significant economic effects on the production of geese. Current commercially available vaccines only rarely prevent GPV infection. In our study, Lactobacillus plantarum NC8 was selected as a vector to express the VP2 gene of GPV, and recombinant L. plantarum pSIP409-VP2/NC8 was successfully constructed. The molecular weight of the expressed recombinant protein was approximately 70 kDa. Mice were immunized with a 2×109 CFU/200 μL dose of the strain, and the ratios and numbers of CD11c+, CD3+CD4+, CD3+CD8+, and IFN-γ- and TNF-α-expressing spleen lymphocytes in the pSIP409-VP2/NC8 group were higher than those in the control groups. In addition, we assessed the capacity of L. plantarum SIP409-VP2/NC8 to induce secretory IgA production. We conclude that administered pSIP409-VP2/NC8 leads to relatively extensive cellular responses. This study provides information on GPV infection and offers a clear framework of available options for GPV control strategies.
Keywords: Lactobacillus plantarum, VP2 gene, goose parvovirus, immunization

© 2016 The Korean Society of Veterinary Science.