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Extensive characterization of feline intra-abdominal adipose-derived mesenchymal stem cells
Hee-Ryang Kim1,†, Jienny Lee1,†, Jeong Su Byeon1, Na-Yeon Gu1, Jiyun Lee2, In-Soo Cho1, Sang-Ho Cha1,*
1Viral Disease Division, Animal and Plant Quarantine Agency, Gimcheon 39660, Korea
2Gangnam Animal Hospital, Pyeongtaek 17982, Korea
Correspondence to: Tel: +82-54-912-0805; Fax: +82-54-912-0812; E-mail: virusmania@korea.kr
The first authors contributed equally to this work.
Received: November 3, 2015; Revised: March 15, 2016; Accepted: June 8, 2016; Published online: July 25, 2016.
Abstract
Mesenchymal stem cells (MSCs) have been isolated from various tissues and well characterized for therapeutic application to clinical diseases. However, in contrast to MSCs from other animal species, the characteristics of feline MSCs have not been well documented. In this study, we attempted to conduct extensive characterization of feline adipose tissue-derived MSCs (fAD-MSCs). fAD-MSCs were individually isolated from the intra-abdominal adipose tissues of six felines. The expression levels of cell surface markers and pluripotent markers were evaluated. Next, the proliferation capacity was analyzed by cumulative population doubling level (CPDL) and doubling time (DT) calculation assays. Differentiation potentials into mesodermal cell lineages of fAD-MSCs were further analyzed by specific staining and molecular markers. All of fAD-MSCs positively expressed cell surface markers such as CD29, CD44, CD90, CD105, CD166, and MHC-I, while CD14, CD34, CD45, and CD73 were negatively expressed. The CPDL of the fAD-MSCs was maintained until passage 5 to 6 (P5 to P6) and DT increased after P5 to P6. Also, stem cell specific pluripotent markers (Oct3/4, Nanog, and SSEA-4) were detected. Importantly, all of the fAD-MSCs demonstrated mesodermal differentiation capacity. These results suggest that well characterized fAD-MSCs could be beneficial, when considering these cells for researches of feline diseases.
Keywords: adipose-derived mesenchymal stem cells, differentiation, feline, multipotent, proliferation


© 2016 The Korean Society of Veterinary Science.