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Chimeric rabies glycoprotein with a transmembrane domain and cytoplasmic tail from Newcastle disease virus fusion protein incorporates into the Newcastle disease virion at reduced levels
Gui Mei Yu, Shu Long Zu, Wei Wei Zhou, Xi Jun Wang, Lei Shuai, Xue Lian Wang, Jin Ying Ge*, Zhi Gao Bu*
Key Laboratory of Veterinary Public Health of Ministry of Agriculture, State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150001, China
Correspondence to: Tel: +86-189-460-6253; Fax: +86-451-5199-7166; E-mail: gjy2003@163.com (JY Ge), zgbu@yahoo.com (ZG Bu)
Received: October 27, 2015; Revised: April 29, 2016; Accepted: July 21, 2016; Published online: August 10, 2016.
Rabies is still an important world-wide health problem. Newcastle disease virus (NDV) has been developed as a vaccine vector in animals using a reverse genetics system. Previously, our group generated a recombinant NDV (LaSota strain) expressing the complete rabies G protein (RVG), named rL-RVG. In this study, we constructed a variant, rL-RVGTM, which expresses a chimeric rabies G protein RVGTM containing the ectodomain of RVG and the transmembrane domain(TM) and cytoplasmic tail (CT) from the NDV fusion glycoprotein to study the function of RVG’s TM and CT. The RVGTM did not detectably incorporate into NDV virions though it was abundantly expressed at the surface of infected BHK-21 cells. The rL-RVG and rL-RVGTM induced similar levels of NDV virus neutralizing antibody (VNA) after initial and secondary vaccination in mice, whereas the rabies virus VNA induction by rL-RVGTM was far lower than that of rL-RVG. Though rL-RVG could spread from cell to cell like rabies virus, rL-RVGTM lost this ability and spread in a manner similar to parental NDV. Our data suggest that the TM and CT of RVG are essential for its incorporation into NDV virions and in the spreading of the recombinant virus from the initially infected cells to surrounding cells.
Keywords: Chimeric rabies glycoprotein, Newcastle disease virus, Rabies virus, Viral vector, Antibody response

© 2016 The Korean Society of Veterinary Science.