Sequence analysis of the spike gene of porcine epidemic diarrhea virus isolated from South China during 2011?2015
Xiaoya Zhao1, Zhili Li2, Xiduo Zeng3, Guanqun Zhang2, Jianqiang Niu2, Baoli Sun1, Jingyun Ma1,*
1College of Animal Science, South China Agricultural University, Guangzhou 510642, China
2Henan University of Science and Technology, Luoyang 471900, China
3Guangdong Wen’s Foodstuff Group Co., Ltd., Yunfu 527400, China
Correspondence to: Tel: +86-20-85280283; Fax: +86-20-85280283; email: majy2400@scau.edu.cn
Received: December 28, 2015; Revised: June 25, 2016; Accepted: July 21, 2016; Published online: August 10, 2016.
Abstract
The spike (S) gene of porcine epidemic diarrhea virus (PEDV) was sequenced from 55 South China field strains isolated from pigs with symptoms of diarrhea. These sequences were compared within the set as well as with the reference strains available in GenBank. Within the 55 South China PEDV field strains, the deduced amino acid sequence identities ranged from 93.8?99.9 % and 90.7?99.5 % when compared with the foreign reference strains reported in GenBank. Our phylogenetic analysis showed that 10 of the 55 South China PEDV strains belonged to G1b and 45 belonged to G2b.
Keywords: Molecular characterization, phylogenetic analysis, porcine epidemic diarrhea virus (PEDV)


© 2016 The Korean Society of Veterinary Science.