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Surveyor assay to diagnose persistent M?llerian duct syndrome in miniature Schnauzers
Young June.Kim1,*, Hyuk Jin Kwon2, Hyuk Soo Byun2, Donguk Yeom2, Jea-Hong Choi2, Joong-Hyun Kim3, Hosup Shim1,3,4,**
1Department of Nanobiomedical Science and BK21 PLUS NBM Global Research Center for Regenerative Medicine, Dankook University, Cheonan 31116, Korea
2Dream Animal Hospital, Seoul 05570, Korea
3Institute of Tissue Regeneration Engineering, Dankook University, Cheonan 31116, Korea
4Department of Physiology, Dankook University School of Medicine, Cheonan 31116, Korea
Correspondence to: Tel: +82 41 550 3865; Fax: +82-41 559 7839; E-mail: shim@dku.edu
Received: January 4, 2016; Revised: May 3, 2016; Accepted: July 21, 2016; Published online: August 10, 2016.
Abstract
Persistent M?llerian duct syndrome (PMDS) is an inherited pseudohermaphroditism in males characterized by the presence of M?llerian duct derivatives. As PMDS dogs often lack clinical symptoms, a molecular diagnosis is essential to identify the syndrome in these animals. In this study, a new molecular method using the DNA mismatch-specific Surveyor nuclease was developed. The Surveyor assay identified the AMHR2 mutation causing PMDS in the Miniature Schnauzer as accurate as the conventional method based on restriction digestion. In addition to the current molecular diagnosis, the new method may benefit increased accuracy in detecting PMDS.
Keywords: Miniature Schnauzers; M?llerian; PMDS; Surveyor


© 2016 The Korean Society of Veterinary Science.