Efficacy test of horse chestnut leaf extract (ALH-L1005) as matrix metalloproteinase inhibitors in ligature-induced periodontitis in canine model
Se Eun Kim1, Tae Hyun Kim1, Shin Ae Park1, Won Tae Kim1, Young Woo Park1, Jae Sang Ahn1, Manbok Jeong1, Min-Young Kim2, Kangmoon Seo1,*
1Department of Veterinary Surgery, College of Veterinary Medicine, Seoul National University, Seoul, Korea
2AngioLab, Inc. Daejeon, Korea
Correspondence to: Tel: +82-880-1258; Fax: +82-2-884-8651; E-mail: kmseo@snu.ac.kr (K Seo), mykim@angiolab.co.kr (MY Kim)
Received: February 11, 2016; Revised: April 28, 2016; Accepted: July 21, 2016; Published online: August 10, 2016.
Abstract
Matrix metalloproteinases (MMPs) are the main proteinase associated with periodontal tissue destruction and remodeling. Therefore, inhibition of the host-derived MMPs is accounted to play a key role in the prevention and reduction of the periodontitis progression. Horse chestnut (Aesculus hippocastanum L.) extracts had been used as a remedy for inflammatory disease, traditionally. This study was performed to assess the clinical effect of ALH-L1005 on the periodontitis as MMPs inhibitor. ALH-L1005 was obtained from horse chestnut leaf and the MMPs inhibitory activities were estimated. Periodontitis was induced in beagles which were assigned to 4 groups and medicated during 6 weeks: LT (ALH-L1005, 100 mg/kg/day), HT (ALH-L1005, 200 mg/kg/day), PC (doxycycline, 10 mg/kg/day), and NC (placebo). Before and after the administration, clinical indices of the teeth and MMPs quantity of gingival tissues using zymography were measured. Clinical conditions of LT, HT and PC groups were significantly improved after 6-week medication. In zymographic evaluations, gelatinolytic and caseinolytic activities were suppressed in LT, HT and PC groups compared to NC groups. These results suggest that ALH-L1005 could be an effective agent for prevention and treatment of periodontitis clinically by inhibiting the activity of gelatinase and collagenase which can detach periodontal ligaments from alveolar bone.
Keywords: dog, doxycycline, horse chestnut leaf extract, matrix metalloproteinase, periodontal disease


© 2016 The Korean Society of Veterinary Science.