Neuronal maturation in the hippocampal dentate gyrus via chronic oral administration of Artemisa annua extract is independent of COX-2 signaling pathway in the diet-induced obesity (DIO) mice model
Hye Kyung Baek1,†, Pan Soo Kim2,†, Ji Ae Song1, Dong-Hwa Choi2, Do Eun Kim1, Seung Il Oh3, Sang-Kyu Park3, Sung-Jo Kim4, Ki-Duk Song5, In Koo Hwang6, Hyung Seok Seo7,Sun Shin Yi1,*
1Department of Biomedical Laboratory Science, College of Medical Sciences, Soonchunhyang University, Asan 31538, Korea
2Biocenter, Gyeonggi Institute of Science and Technology Promotion (GSTEP), Suwon 16229, Korea
3Deparment of Medical Biotechnology, College of Medical Sciences, Soonchunhyang University, Asan 31538, Korea
4Department of Biotechnology, Hoseo University, Asan 31499, Korea
5Department of Animal Biotechnology, College of Agricultural Life Science, ChonBuk National University, Jeonju 54896, Korea
6Department of Anatomy and Cell Biology, College of Veterinary Medicine, Seoul National University, Seoul 08826, Korea
7Department of Health Science, Konyang University, Nonsan 32992, Korea
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The first two authors contributed equally to this work.
Received: March 17, 2016; Revised: June 21, 2016; Accepted: July 21, 2016; Published online: August 10, 2016.
Abstract
Recently, we reported that Artemisia annua (AA) has anti-adipogenic properties in vitro and in vivo. Reduction of adipogenesis by AA treatment may dampen systemic inflammation and protect neurons from cytokine-induced damage. Therefore, the present study was performed to assess whether AA increases neuronal maturation by reducing inflammatory responses, such as those mediated by COX-2. Mice were fed normal chow or a high-fat diet with or without chronic daily oral administration of AA extract (0.2g/10mL/kg) for 4 weeks; then, changes in their hippocampal dentate gyri were measured with immunohistochemistry/immunofluorescence staining for BrdU, DCX, and NeuN, markers of neuronal maturation, and a quantitative Western blot for COX-2 and Iba-1, in order to assess correlations between systemic inflammation (Il-6) and food type. Additionally, we tested the effect of AA in an Alzheimer’s disease model of C. elegans and uncovered a potential benefit. In the present study, we show that chronic AA dosing significantly increases neuronal maturation, particularly in the high-fat diet group. This effect was seen in the absence of any changes in COX-2 levels in mice given the same type of food, pointing to the possibility of alternate anti-inflammatory pathways in the stimulation of neurogenesis and neuro-maturation in a background of obesity.
Keywords: Artemisia annua; anti-obesity; adipogenesis; neurogenesis; neuro-maturation; COX-2


© 2016 The Korean Society of Veterinary Science.