Bovine adenovirus type 3 virions cannot be rescued in vivo post full-length viral genome transfection in the absence of detectable polypeptide IX
Peng Zhang1,†, Qinghong Xue2,†, Jing Ma1, Jingjing RenN 1, Shuili Xia1, Lu Zhang1, Wenbin Wang1 , Supesh K. Tikoo3,4,*, Enqi Du1,*
1College of Veterinary Medicine, North-west A&F University, Yangling, Shaanxi, China
2Chinese Institute of Veterinary drug controls, Beijing, China
3VIDO-InteVac, University of Saskatchewan Saskatoon, Saskatchewan, Canada
4Vaccinology & Immunotherapeutics program, School of Public Health, University of Saskatchewan, Saskatoon, Saskatchewan, Canada
Correspondence to: Tel: +86-29-87091106; Fax: +86-29-87091032; E-mail: duenqi227@126.com (E Du), suresh.tik@usask.ca (S Tikoo)
The first two authors contributed equally to this work.
Received: November 18, 2015; Revised: May 30, 2016; Accepted: August 26, 2016; Published online: September 1, 2016.
Abstract
Bovine adenovirus type 3 (BAdV-3) is being developed as potential vehicles for vaccination. To this end, a more comprehensive understanding of BAdV-3 biology is essential. Here we focused on the role of pIX in BAdV-3 virions rescue post BAdV-3 genome transfection. Initially, pIX deletion or initiation codon mutation abolished the production of progeny virions suggested that pIX was essential for the rescue of BAdV-3 containing full-length genome. Moreover, a panel of mutant pIX, we confirmed that the conserved N-terminus and putative leucine zipper element (PLZP) were the essential domains, while the C-terminus following coiled-coil domain was non-essential. In addition, the swap of PLZP element and following region of BAdV-3 pIX to corresponding domains of human adenovirus type 5 (HAdV-5) did not affect virions production while the swap of entire pIX abolished the production of progeny virions. Thus, we suggested that the failure of full-length pIX swap might be due to the species specificity of the N-terminus.
Keywords: adenovirus, bovine adenovirus type 3, polypeptide IX, viral rescue


© 2016 The Korean Society of Veterinary Science.