Received: December 3, 2015; Revised: May 26, 2016; Accepted: August 26, 2016; Published online: September 1, 2016.
The efficacy of six commercial disinfectants were evaluated using Salmonella enterica serovar Typhimurium under simulated natural conditions such as sub-zero temperature, short disinfecting time, and types of surface structure (uneven or smooth). We used a suspension-model test to determine the disinfecting efficacy under varying contact times (1, 5, 10, and 30 min) and temperatures (25°C, 4°C, 0°C, and -10°C). The bactericidal effect according to the surface structure was measured using a carrier-model test at 25°C and -10°C. Moreover, the effective concentrations of each disinfectant were fixed giving a disinfecting effect within a short time (<1 min) at 25°C and -10°C. The suspension-model experiment revealed that bactericidal efficacy significantly dropped at low temperature for most of the disinfectants used. In contrast, a product of sodium dichloroisocyanurate showed the strongest efficacy. In the carrier test, bacterial load on wooden surface was more difficult to be removed than on stainless steel surface. Thus, commercial disinfectant products vary in their disinfecting efficacy, which is affected by several field factors including temperature, contact time, and bacterial carrier materials, and environmental conditions and surface for disinfection should be considered prior to selecting an optimal disinfectant in the field.