Efficacy evaluation of commercial disinfectants by using Salmonella enterica serovar Typhimurium as a test organism
Yangho Jang1, Kwangjick Lee1, Seonjong Yun1, Myoungheon Lee1, Jaeyoung Song1, Byungjoon Chang2, Nong-hoon Choe2,*
1Animal and Plant Quarantine Agency (QIA), Anyang, Gyeonggi-do 430-757, Korea
2College of Veterinary Medicine, Konkuk University, Seoul 143-701, Korea
Correspondence to: Tel: +82-2-450-3709; Fax: +82-2-454-3709; E-mail: nojamaji@hanmail.net
Received: December 3, 2015; Revised: May 26, 2016; Accepted: August 26, 2016; Published online: September 1, 2016.
Abstract
The efficacy of six commercial disinfectants were evaluated using Salmonella enterica serovar Typhimurium under simulated natural conditions such as sub-zero temperature, short disinfecting time, and types of surface structure (uneven or smooth). We used a suspension-model test to determine the disinfecting efficacy under varying contact times (1, 5, 10, and 30 min) and temperatures (25°C, 4°C, 0°C, and -10°C). The bactericidal effect according to the surface structure was measured using a carrier-model test at 25°C and -10°C. Moreover, the effective concentrations of each disinfectant were fixed giving a disinfecting effect within a short time (<1 min) at 25°C and -10°C. The suspension-model experiment revealed that bactericidal efficacy significantly dropped at low temperature for most of the disinfectants used. In contrast, a product of sodium dichloroisocyanurate showed the strongest efficacy. In the carrier test, bacterial load on wooden surface was more difficult to be removed than on stainless steel surface. Thus, commercial disinfectant products vary in their disinfecting efficacy, which is affected by several field factors including temperature, contact time, and bacterial carrier materials, and environmental conditions and surface for disinfection should be considered prior to selecting an optimal disinfectant in the field.
Keywords: Salmonella, carrier-model test, disinfectant, low temperature, surface structure, suspension-model test


© 2016 The Korean Society of Veterinary Science.