Effect of phosphorus deficiency on erythrocytic morphology and function of cows
Ziwei Zhang1, Mingyu Bi2, Jie Yang1, Haidong Yao1, Zhonghua Liu3,*, Shiwen Xu1,*
1College of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, P. R. China
2Harbin Railway Public Security Bureau police dog base, Harbin 150056, P. R. China
3College of Life Science, Northeast Agricultural University, Harbin 150030, China
Correspondence to: Tel: +86 451 55190407, E-mail: liu086@126.com (Zhonghua Liu), shiwenxu@neau.edu.cn (Shi-wen Xu)
Received: January 4, 2016; Revised: June 30, 2016; Accepted: August 26, 2016; Published online: September 1, 2016.
Abstract
The aim of this study was to evaluate the influence of P deficiency on the morphological and functional characteristics of erythrocytes in cows. Forty Holstein-Friesian dairy cows in mid-lactation were randomly divided into two groups of 20 each and were fed either a low-P diet (0.03% P/kg DM) or a control diet (0.36% P/kg DM). Red blood cell indices results showed RBC and MCH decreased while MVC increased significantly (P<0.05) with P deficiency in cows; erythrocyte morphology showed erythrocyte destruction had occurred in P deficiency cows. Erythrocytes’ functional characteristics results showed T-Bil and I-Bil concentrations and the activities of AST and ALT in the serum of the P-deficient diet were increased significantly than those of the control diet. Activities of SOD and GSH-Px in erythrocytes were lower, while the MDA content in blood of cows on the P-deficient diet was greater than that in control diet. Na+/K+-ATPase and Mg2+-ATPase activities were decreased in the P-deficient diet compared to the control diet; however, Ca2+-ATPase activity was not significantly affected. The phospholipid composition of the erythrocyte membrane was changed, and membrane fluidity rigidified in the P-deficient diet. The results indicated P deficiency might impair erythrocyte integrity and functional characteristics in cows.
Keywords: cow, erythrocyte, morphology and function, phosphorus deficiency


© 2016 The Korean Society of Veterinary Science.