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Guanylyl cyclase C and guanylin reduce fat droplet accumulation in cattle mesenteric adipose tissue
Masahiro Yasuda1,*, Jyunya Kawabata1, Sayaka Akieda-Asai2, Tetsuo Nasu1, Yukari Date2
1Department of Veterinary Anatomy, Faculty of Agriculture, University of Miyazaki, Miyazaki 889-2192, Japan
2Frontier Science Research Center, University of Miyazaki, Miyazaki 889-1692, Japan
Correspondence to: Tel/Fax: +81-985-58-7264; E-mail: yasudaja@cc.miyazaki-u.ac.jp
Received: January 20, 2016; Revised: June 28, 2016; Accepted: August 26, 2016; Published online: September 1, 2016.
Abstract
Guanylyl cyclase C (GC-C) is a member of a family of enzymes that metabolize GTP to cGMP and was first identified as a receptor for heat-stable enterotoxin. Guanylin (GNY) has since been identified as an endogenous ligand for GC-C in the intestine of several species in mammals. The GNY/GC-C system regulates ion transportation and pH in the mucosa. Recently, it was reported that GC-C and GNY are involved in lipid metabolism in rat mesenteric adipose tissue macrophages. To examine the role of GC-C and GNY in lipid metabolism in cattle, we used a bovine mesenteric adipocyte primary culture system and a coculture system for bovine adipocytes and GNY-/GC-C-expressing macrophages. Fat droplets were found to accumulate in bovine mesenteric adipocytes cultured alone, whereas in adipocytes indirectly cocultured with macrophages, very few fat droplets accumulated. We also found that GC-C was present in bovine mesenteric adipose tissue and that fat droplet accumulation decreased after in vitro GNY administration. Expression of mRNAs encoding lipogenic factors decreased significantly in adipocytes after either coculture or GNY administration. These data suggest that the GNY/GC-C system is part of the control of lipid accumulation in bovine mesenteric adipose tissue.
Keywords: cattle, guanylin, guanylyl cyclase C, lipogenic factor, mesenteric adipose tissue


© 2016 The Korean Society of Veterinary Science.