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A survey of porcine respiratory disease complex (PRDC) associated pathogens among commercial pig farms of Korea via oral fluid method
Yeotaek Cheong1, Changin Oh1, Kunkyu Lee1, Ki-Hyun Cho2,*
1Department of Infectious Diseases, College of Veterinary Medicine, Konkuk University, 120 Neungdong-ro, Gwangjin-gu, Seoul 05029, Korea
2Center for AI control and Prevention, Animal and Plant Quarantine Agency, 177 Hyeoksin8-ro, Gimcheon, Gyeongsangbuk-do, 39660, Korea
Correspondence to: Tel: +82-54-912-0389; Fax: +82-54-912-1083; E-mail: vet10@naver.com
Received: March 10, 2016; Revised: July 12, 2016; Accepted: August 26, 2016; Published online: September 1, 2016.
Abstract
Oral fluid analysis for monitoring herd is an interest in commercial pig farms in Korea. The aim of this study was to investigate the positive rates and correlation of eight pathogens associated with porcine respiratory disease complex (PRDC) by analyzing oral fluid samples from 214 pig groups from 56 commercial farms. The samples collected by rope-chewing method were analyzed with reverse-transcription polymerase chain reaction (RT-PCR) or standard PCR depending on microorganisms. The pathogens were divided into virus group and bacteria group. The former consisted of porcine reproductive and respiratory syndrome virus (PRRSV) and porcine circovirus type 2 (PCV2), and the latter Pasteurella multocida (PM), Haemophilus parasuis (HPS), Actinobacillus pleuropneumoniae (APP), Mycoplasma hyopneumoniae (MHP), Mycoplasma hyorhinis (MHR), and Streptococcus suis (SS). All pathogens were detected more than once in the PCR reaction. Analysis based on age distribution showed increasing PCR positive rates for PCV2 and MHP with increasing age. SS was inverse proportion. Correlations between pathogens were assessed in 36 different combination pairs. Seven pairs showed statistically significant correlations. In conclusion, the oral fluid method could be a feasible way to detect various swine respiratory disease pathogens and therefore, could complement current monitoring systems for respiratory diseases in the swine industry.
Keywords: Korea, oral fluid, pathogens, polymerase chain reaction, porcine respiratory disease complex


© 2016 The Korean Society of Veterinary Science.