J. Vet. Sci. 2016; 17(4): 479-487  https://doi.org/10.4142/jvs.2016.17.4.479
Development and evaluation of an immunochromatographic assay using a gp51 monoclonal antibody for the detection of antibodies against the bovine leukemia virus
Eun-Ju Kim1, Kwang-Myun Cheong2, Ha-Kyung Joung1, Bo-Hye Kim1, Jae-Young Song3, In-Soo Cho1, Kyoung-Ki Lee4, Yeun-Kyung Shin1,*
Divisions of 1Viral Disease, 3Veterinary Drugs and Biologics, and 4Animal Disease Diagnostic, Animal and Plant Quarantine Agency, Anyang 14086, Korea
2Research Institution, MEDIAN Diagnostics Inc., Chuncheon 24399, Korea
Correspondence to: Yeun-Kyung Shin
Tel: +82-31-467-1827; Fax: +82-31-467-1797;
E-mail: shinyk2009@korea.kr
Received: August 24, 2015; Revised: January 6, 2016; Accepted: February 22, 2016; Published online: December 30, 2016.
Abstract
Infection of cattle with bovine leukemia virus (BLV) has been observed and reported worldwide, including in Korea. The onsite identification of infected cattle would help decreasing and eradicating BLV infections on farms. Here, we present a new immunochromatographic assay that employs monoclonal antibodies (MAbs) for the detection of antibodies against BLV in the field. BLV envelope glycoprotein (gp)51 was expressed in E. coli, and MAbs against recombinant BLV gp51 were generated for the development of an immunochromatographic assay to detect BLV antibodies in cattle. The sensitivity and specificity of the assay were determined by comparing these results with those obtained from a standard enzyme linked immunosorbent assay (ELISA). A total of 160 bovine sera were used to evaluate the new immunochromatographic assay. Using ELISA as a reference standard, the relative specificity and sensitivity of this assay were determined to be 94.7% and 98%, respectively. Because of its high sensitivity and specificity, this BLV antibody detection assay would be suitable for the onsite identification of BLV infection in the field.
Keywords: antibody detection, bovine leukemia virus, immunochromatography


© 2016 The Korean Society of Veterinary Science.