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J. Vet. Sci. 2016; 17(4): 497-503  https://doi.org/10.4142/jvs.2016.17.4.497
Genomic diversity of the Avian leukosis virus subgroup J gp85 gene in different organs of an infected chicken
Fanfeng Meng1,†, Xue Li1,2,†, Jian Fang1,2, Yalong Gao2, Lilong Zhu2, Guiju Xing2, Fu Tian2, Yali Gao2, Xuan Dong1, Shuang Chang1, Peng Zhao1, Zhizhong Cui1,*, Zhihao Liu1
1Shandong Agricultural University, Taian 271018, China
2Beijing Dafaun Poultry Breeding Company Ltd., Beijing 10010, China
Correspondence to: Zhizhong Cui
Tel: +86-538-8241560; Fax: +86-538-8241419;
E-mail: zzcui@sdau.edu.cn
Received: January 21, 2016; Revised: April 24, 2016; Accepted: June 8, 2016; Published online: December 30, 2016.
Abstract
The genomic diversity of Avian leukosis virus subgroup J (ALV-J) was investigated in an experimentally infected chicken. ALV-J variants in tissues from four different organs of the same bird were re-isolated in DF-1 cells, and their gp85 gene was amplified and cloned. Ten clones from each organ were sequenced and compared with the original inoculum strain, NX0101. The minimum homology of each organ ranged from 96.7 to 97.6%, and the lowest homology between organs was only 94.9%, which was much lower than the 99.1% homology of inoculum NX0101, indicating high diversity of ALV-J, even within the same bird. The gp85 mutations from the left kidney, which contained tumors, and the right kidney, which was tumor-free, had higher non-synonymous to synonymous mutation ratios than those in the tumor-bearing liver and lungs. Additionally, the mutational sites of gp85 gene in the kidney were similar, and they differed from those in the liver and lung, implying that organ- or tissue-specific selective pressure had a greater influence on the evolution of ALV-J diversity. These results suggest that more ALV-J clones from different organs and tissues should be sequenced and compared to better understand viral evolution and molecular epidemiology in the field.
Keywords: Avian leukosis virus subgroup J, genomic diversity, glycoprotein 85, mutation


© 2016 The Korean Society of Veterinary Science.