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Genetic diversity and phylogenetic analysis of Porcine reproductive and respiratory syndrome virus in southern China from 2007 to 2014
Qiwen Wu1, Zhili Li2, Guanqun Zhang3, Jianqiang Niu3, Xiduo Zeng3, Baoli Sun1, Jingyun Ma1,*
1College of Animal Science, South China Agricultural University, Guangzhou, 510642, China
2Henan University of Science and Technology, Luoyang, China
3Guangdong Wen’s Foodstuff Group Co., Ltd., Xinxing, 527400, China
Correspondence to: Tel/Fax: +86-20-8528-0283; E-mail: majy2400@scau.edu.cn
Received: February 15, 2016; Revised: July 20, 2016; Accepted: November 23, 2016; Published online: January 4, 2017.
Abstract
Porcine reproductive and respiratory syndrome virus (PRRSV) has a high degree of genetic variation. Here, we characterized the genetic variation and evolutionary relationships among circulating PRRSV strains in Southern China. In this study, we analyzed 29 NSP2 strains and 150 ORF5 strains from clinical samples collected in Southern China during 2007-2014. The alignment results showed that the nucleotide identities of the two genes among these strains were 80.5-99.7% and 80.9-100%, respectively. Phylogenetic analysis based on NSP2 gene showed that high pathogenic PRRSV were still the dominant virus in Southern China from 2013 to 2014. Compared with reference strains CH-1a and VR-2332, the field strain 131101-GD-SHC, which shared high homology with JXA1-P170, had a novel 12 amino acid deletion at position 499-510. Phylogenetic analysis based on the ORF5 gene showed that HP-PRRSV, VR2332-like strains, and QYYZ-like strains were simultaneously circulating in Southern China from 2007 to 2014, suggesting that the type 2 PRRSV was more diverse in recent years in Southern China. In conclusion, mutations in the decoy epitope and primary neutralizing epitope could be markers of viral evolution used to study the evolutionary relationships between PRRSV strains in China.
Keywords: GP5, NSP2, PRRSV, phylogenetic analysis


© 2017 The Korean Society of Veterinary Science.