Development of an immunochromatographic strip for the detection of antibodies against porcine reproductive and respiratory syndrome virus
Huawei Li1,5,†, Jifei Yang2,†, Dengke Bao2, Jie Hou2, Yubao Zhi2, Yanyan Yang2, Pengchao Ji1, Enmin Zhou1, Songlin Qiao2,*, Gaiping Zhang1,3,4,*
1College of Veterinary Medicine, Northwest A&F University, Yangling 712100, China
2The Provincial Key Laboratory of Animal Immunology, and 3College of Veterinary Medicine,Henan Academy of Agricultural Sciences, Zhengzhou 450002, China
4Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonosis, Yangzhou, China
5The Bioengineering Department, Henan University of Animal Husbandry and Economy, Zhengzhou 450046, China
Correspondence to: Tel: +86-371-63550369; Fax: +86-371-63558998; E-mail: zhanggaiping2003@163.com (GP Zhang), cdj565@gmail.com (S Qiao)
The first two authors contributed equally to this work.
Received: April 18, 2016; Revised: July 22, 2016; Accepted: November 23, 2016; Published online: January 4, 2017.
Abstract
A simple and rapid immunochromatographic test strip using a colloidal gold-labeled recombinant Nsp7 antigen probe was successfully developed for the detection of anti-PRRSV antibodies in swine. Recombinant Nsp7 protein of PRRSV labeled with colloidal gold was dispensed on a conjugate pad as the detector. Staphylococcal protein A (SPA) and purified porcine anti-Nsp7 antibodies were blotted on a nitrocellulose membrane for the test and control lines, respectively. A comparison between the new assay and standard diagnostic tests enzyme-linked immunosorbent assays (ELISA) and immunoperoxidase monolayer assay (IPMA) was also performed. The immunochromatographic test strip was shown to be of high specificity and sensitivity. Furthermore, the strip assay is rapid and easy to perform with no requirement of professional skills, nor equipment. It is suggested that the immunochromatographic test strip can be used to quickly and accurately detect PRRSV antibody and to be suitable for diagnosis in the field.
Keywords: ELISA, immunochromatographic strip, porcine reproductive and respiratory syndrome


© 2017 The Korean Society of Veterinary Science.