Molecular cloning and characterization of the porcine ribosomal protein L21
Wu-Sheng Sun1,2, Ju-Lan Chun2, Dong-Hwan Kim1, Jin-Seop Ahn1, Min-Kyu Kim2, In-Sul Hwang3, Dae-Jin Kwon3, Seong-Soo Hwang3 , Jeong-Woong Lee1,*
1Biotherapeutics Translational Research Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon 305-806, Korea
2Department of Animal Science and Biotechnology, College of Agriculture and Life Science, Chungnam National University, Daejeon 305-806, Korea
3Animal Biotechnology Division, National Institute of Animal Science, Wanju 565-851, Korea
Correspondence to: Tel: +82-42-860-4428; Fax: +82-42-860-4608; E-mail: jwlee@kribb.re.kr
Received: May 13, 2016; Revised: October 12, 2016; Accepted: November 23, 2016; Published online: January 4, 2017.
Abstract
Ribosomal protein L21 (RPL21) is a structural component of the 60S subunit of the eukaryotic ribosome. This protein plays an important role in protein synthesis and the occurrence of hereditary diseases. Pig is a common laboratory model, however, to the best of our knowledge, its RPL21 gene has not been cloned to date. In this study, we cloned and identified the full-length sequence of the pig RPL21 gene for the first time. Then we studied its expression pattern and function by overexpression or knockdown approach. As a result, we obtained a 604-bp segment that contains a 483-bp open reading frame encoding 160 amino acids. We found the pig RPL21 gene is located in the “+” strand of chromosome 11, which spans 2167 bp from 4199792 to 4201958. Pig RPL21 protein has nine strands and two helices in its secondary structure. Pig RPL21 is predominantly expressed in the ovary and lung compared to the kidney, small intestine and skin but expressed at lower levels in the heart and liver. Furthermore, we found RPL21 expression level is closely connected with cell proliferation and cell cycle arrest. These results are intended to provide valid information for the further study of pig RPL21.
Keywords: Sus scrofa, conservation, gene expression, molecular cloning, ribosomal protein L21,


© 2017 The Korean Society of Veterinary Science.