J. Vet. Sci. 2017; 18(1): 39-49  
Inflammation affects the viability and plasticity of equine mesenchymal stem cells: possible implications in intra-articular treatments
Laura Barrachina1,2, Ana Rosa Remacha1, Antonio Romero1,2, Francisco Jos? V?zquez1,2, Jorge Albareda1,3, Marta Prades1,4, Beatriz Ranera1, Pilar Zaragoza1, Inmaculada Mart?n-Burriel1, Clementina Rodellar1,*
1Laboratory of Biochemical Genetics LAGENBIO, Veterinary Hospital, University of Zaragoza, 50013 Zaragoza, Spain
2Service of Equine Surgery and Medicine, Veterinary Hospital, University of Zaragoza, 50013 Zaragoza, Spain
3Service of Orthopedic Surgery and Traumatology, University Clinical Hospital Lozano Blesa, 50009 Zaragoza, Spain
4Service of Equine Surgery, Veterinary Hospital, Autonomous University of Barcelona, 08193 Barcelona, Spain
Correspondence to: Clementina Rodellar
Tel: +34-976-761622; Fax: +34-976-762949; E-mail: rodellar@unizar.es
Received: January 21, 2016; Revised: March 19, 2016; Accepted: May 12, 2016; Published online: March 30, 2017.
Mesenchymal stem cells (MSCs) are gaining relevance for treating equine joint injuries because of their ability to limit inflammation and stimulate regeneration. Because inflammation activates MSC immunoregulatory function, proinflammatory priming could improve MSC efficacy. However, inflammatory molecules present in synovial fluid or added to the culture medium might have deleterious effects on MSCs. Therefore, this study was conducted to investigate the effects of inflammatory synovial fluid and proinflammatory cytokines priming on viability and plasticity of equine MSCs. Equine bone marrow derived MSCs (eBM-MSCs) from three animals were cultured for 72 h in media supplemented with: 20% inflammatory synovial fluid (SF); 50 ng/mL IFN-γ and TNF-α (CK50); and 20 ng/mL IFN-γ and TNF-α (CK20). Proliferation assay and expression of proliferation and apoptosis-related genes showed that SF exposed-eBM-MSCs maintained their viability, whereas the viability of CK primed-eBM-MSCs was significantly impaired. Tri-lineage differentiation assay revealed that exposure to inflammatory synovial fluid did not alter eBM-MSCs differentiation potential; however, eBM-MSCs primed with cytokines did not display osteogenic, adipogenic or chondrogenic phenotype. The inflammatory synovial environment is well tolerated by eBM-MSCs, whereas cytokine priming negatively affects the viability and differentiation abilities of eBM-MSCs, which might limit their in vivo efficacy.
Keywords: horses, mesenchymal stromal cells, joint diseases, proinflammatory cytokines, synovial fluid

© 2017 The Korean Society of Veterinary Science.