J. Vet. Sci. 2017; 18(1): 51-58  
Molecular-level evaluation of selected periodontal pathogens from subgingival regions in canines and humans with periodontal disease
Magdalena Goły?ska1,*, Izabela Polkowska1, Małgorzata Bartoszcze-Tomaszewska2, Aleksandra Sobczy?ska-Rak1, Łukasz Matuszewski3
1Department and Clinic of Animal Surgery, Faculty of Veterinary Medicine, University of Life Sciences in Lublin, 20-612 Lublin, Poland
2Dental Center ‘Lardent’, 20-135 Lublin, Poland
3Children’s Orthopaedic Clinic and Rehabilitation Department, Medical University of Lublin, 20-093 Lublin, Poland
Correspondence to: Magdalena Gołyńska
Tel: +48-535353554; Fax: +48-815243808; E-mail: magdareba@wp.pl
Received: December 30, 2015; Revised: March 23, 2016; Accepted: May 12, 2016; Published online: March 30, 2017.
Dogs commonly serve as a model for various human conditions, including periodontal diseases. The aim of this study was to identify the anaerobic bacteria that colonize the subgingival areas in dogs and humans by using rapid real-time polymerase chain reaction (RT-PCR)-based tests and to compare the results obtained in each species. Bacterial microflora evaluations, both quantitative and qualitative, were performed by applying ready-made tests on twelve dogs and twelve humans. Five samples were collected from each subject’s deepest gingival pockets and joined to form a collective sample. The results of the study revealed interspecies similarities in the prevalences of Porphyromonas (P.) gingivalis, Treponema denticola, Tannerella forsythia, and Fusobacterium nucleatum. Red complex bacteria comprised the largest portion of the studied bacterial complexes in all study groups, with P. gingivalis being the most commonly isolated bacterium. The results show similarities in the prevalence of bacterial microflora in dogs and humans. Microbiological analysis of gingival pockets by using rapid real-time PCR-based tests in clinical practice, both veterinary and human, can facilitate the choice of appropriate pharmacological treatment and can provide a basis for subsequent verification of the treatment’s effectiveness.
Keywords: anaerobic bacteria, dogs, humans, periodontal diseases, real-time polymerase chain reaction

© 2017 The Korean Society of Veterinary Science.