J. Vet. Sci. 2017; 18(1): 59-65  https://doi.org/10.4142/jvs.2017.18.1.59
Inhibition by miR-410 facilitates direct retinal pigment epithelium differentiation of umbilical cord blood-derived mesenchymal stem cells
Soon Won Choi1,2,†, Jae-Jun Kim1,2,†, Min-Soo Seo1, Sang-Bum Park1, Tae-Hoon Shin1,2, Ji-Hee Shin1,2, Yoojin Seo1,2,‡,§, Hyung-Sik Kim1,2,‡,§, Kyung-Sun Kang1,2,*
1Adult Stem Cell Research Center, College of Veterinary Medicine, Seoul National University, Seoul 08826, Korea
2Research Institute for Veterinary Science, College of Veterinary Medicine, Seoul National University, Seoul 08826, Korea
Correspondence to: Kyung-Sun Kang
Tel: +82-2-880-1298; Fax: +82-2-876-7610; E-mail: kangpub@snu.ac.kr
Received: October 6, 2015; Accepted: May 12, 2016; Published online: March 30, 2017.
Abstract
Retinal pigment epithelium (RPE) is a major component of the eye. This highly specialized cell type facilitates maintenance of the visual system. Because RPE loss induces an irreversible visual impairment, RPE generation techniques have recently been investigated as a potential therapeutic approach to RPE degeneration. A microRNA-based technique is a new strategy for producing RPE cells from adult stem cell sources. Previously, we identified that antisense microRNA-410 (anti-miR-410) induces RPE differentiation from amniotic epithelial stem cells. In this study, we investigated RPE differentiation from umbilical cord blood-derived mesenchymal stem cells (UCB-MSCs) via anti-miR-410 treatment. We identified miR-410 as a RPE-relevant microRNA in UCB-MSCs from among 21 putative human RPE-depleted microRNAs. Inhibition of miR-410 induces overexpression of immature and mature RPE-specific factors, including MITF, LRAT, RPE65, Bestrophin, and EMMPRIN. The RPE-induced cells were able to phagocytize microbeads. Results of our microRNA-based strategy demonstrated proof-of-principle for RPE differentiation in UCB-MSCs by using anti-miR-410 treatment without the use of additional factors or exogenous transduction.
Keywords: microRNA, miR-410, retinal pigment epithelium, umbilical cord blood-derived mesenchymal stem cells?


© 2017 The Korean Society of Veterinary Science.