J. Vet. Sci. 2017; 18(1): 101-104  
Stage-specific embryonic antigen: determining expression in canine glioblastoma, melanoma, and mammary cancer cells
Weiming Lin1,2,*, Jaime F Modiano1,*, Daisuke Ito1,†
1Department of Veterinary Clinical Sciences and Masonic Cancer Center, University of Minnesota, Minneapolis, MN 55455, USA 2Department of Veterinary Medicine, College of Life Sciences, Longyan University, Longyan 364012, China
Correspondence to: Weiming Lin, Jaime F Modiano
Tel/Fax: +1-612-625-7436; E-mails: modiano@umn.edu (JF Modiano), wmlin925@126.comwmlin925@126.com (W Lin)
Present address: Japan Medical Immuno-Oncology, Bristol-Myers K.K, Tokyo, Japan
Received: December 17, 2015; Revised: April 19, 2016; Accepted: June 8, 2016; Published online: March 30, 2017.
The expression of stage-specific embryonic antigens (SSEAs) was determined in several types of canine cancer cells. Flow cytometry showed SSEA-1 expression in glioblastoma, melanoma, and mammary cancer cells, although none expressed SSEA-3 or SSEA-4. Expression of SSEA-1 was not detected in lymphoma, osteosarcoma, or hemangiosarcoma cell lines. Relatively stable SSEA-1 expression was observed between 24 and 72 h of culture. After 8 days in culture, sorted SSEA-1? and SSEA-1+ cells re-established SSEA-1 expression to levels comparable to those observed in unsorted cells. Our results document, for the first time, the expression of SSEA-1 in several canine cancer cell lines.
Keywords: canine cancer, flow cytometry, magnetic-activated cell sorting, stage-specific embryonic antigens

© 2017 The Korean Society of Veterinary Science.