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Potential biomarkers as the indicator of vertical transmission of Johne’s disease in a Korean native cattle farm
Hong-Tae Park1,†, Hyun-Eui Park1,†, Yong-Il Cho2, Eui-Hyung Kim3, Myunghwan Jung1, Seung Won Shin1, Su-Hyung Lee4, Dae-Yong Kim4, Han Sang Yoo1,*
1Department of Infectious Diseases, College of Veterinary Medicine, Seoul National University, Seoul, 08826, Korea
2Department of Animal Science & Technology, Suncheon National University, Suncheon, 57922, Korea
3National Institute of Animal Science, Rural Development Administration, Pyeongchang, 25340, Korea
4Department of Veterinary Pathology, College of Veterinary Medicine, Seoul National University, Seoul, 08826, Korea
Correspondence to: Tel: +82-2-880-1263; Fax: +82-2-874-2738; E-mail: yoohs@snu.ac.kr
The first two authors contributed equally to this work.
Received: May 25, 2016; Revised: January 19, 2017; Accepted: February 7, 2017; Published online: April 6, 2017.
Abstract
Paratuberculosis (PTB) is caused by Mycobacterium avium subsp. paratuberculosis (MAP) which is one of the most widespread and economically important disease in cattle. Calves are raising with natural breast feeding without separation from their mothers after birth in most Korean native cattle farms (Hanwoo breed). Vertical transmission of the disease has been known even though exact infection route of PTB has not been revealed in Korean native cattle farm. Therefore, calves from MAP sero-positive cows were tested by detection of MAP in the feces and tissues, and antibody against MAP. MAP was detected in the tissues from calves by PCR. From the results, complete eradication of MAP can be possible when the accurate diagnostic methods to detect infected calves in this period have been added to current eradication strategy, such as isolation of new born calves and feeding sterilized colostrum, because these infected individuals are likely to develop into potential fecal shedder at any time. Some genes which were known as prognostic biomarkers of the MAP infection were changed in both of calves and cows (p<0.05). Expression of two genes (HGF and SERPINE1) were decreased in both of MAP infected cattle and their offspring with a significant change (p<0.01). These finding suggest that the gene expression profile of biomarkers might be applied to detect early stage of MAP infection.
Keywords: Mycobacterium avium subsp. paratuberculosis, Korean native cattle, Vertical transmission, Biomarker, Subclinical infection


© 2017 The Korean Society of Veterinary Science.