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Generation, characterization and application in serodiagnosis of recombinant swine vesicular disease viral like particles
Wanhong Xu, Melissa Goolia, Tim Salo, Zhidong Zhang†, Ming Yang*
National Centre for Foreign Animal Disease, 1015 Arlington Street, Winnipeg, Manitoba, Canada R3E 3M4
Correspondence to: Tel: +1-204-789-2103; Fax: +1-204-789-2038; E-mail: Ming.yang@inspection.gc.ca
Current address: Lanzhou Veterinary Research Institute Chinese Academy of Agricultural Sciences (CAAS), No 1, Xujiaping, Lanzhou, Gansu, 730046, P.R. China
Received: July 11, 2016; Revised: December 2, 2016; Accepted: February 22, 2017; Published online: April 6, 2017.
Swine vesicular disease virus (SVDV) is a highly contagious disease that causes vesicular disease in pigs. The importance of the disease is due to its indistinguishable clinical signs from foot–and–mouth disease that prevents international trade of swine and related products. SVD–specific antibody detection using the enzyme–linked immunosorbent assay (ELISA) is the most versatile and commonly used method for SVD surveillance and export certification. The inactivated SVD virus is the commonly used antigen in ELISAs. A recombinant SVD–virus like particle (VLP) was generated using a Bac–to–Bac baculovirus expression system. Analyses of SVD–VLP by electron microscopy, Western blotting, immunofluorescent assay, and mass spectrometry confirmed that the recombinant SVD–VLPs morphologically resemble those of authentic viruses. The SVD–VLP was evaluated as a replacement source of inactivated whole virus in a competitive and isotype–specific ELISAs for the detection of antibodies against SVDV. The recombinant SVD–VLPs produced similar results with inactivated whole virus antigen in both ELISAs. The VLP–cELISA results are comparable to the virus neutralization test for antibody detection from experimentally inoculated pigs with SVDV. The recombinant SVD–VLP offers a safe and valuable alternative to virus antigen for diagnostic assays.
Keywords: Swine vesicular disease virus; Virus–like particle; enzyme–linked immunosorbent assay; Serodiagnosis

© 2017 The Korean Society of Veterinary Science.