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Molecular and genomic features of Mycobacterium bovis strain 1595 isolated from Korean cattle
Narae Kim1,2, Yunho Jang1, Jin Kyoung Kim1, Soyoon Ryoo1, Ka Hee Kwon1, Miso Kim1, Shin Seok Kang4, Hyeon Seop Byeon4, Hee Soo Lee1, Young-Hee Lim3,*, Jae-Myung Kim1,*
1Bacterial Disease Division, Animal and Plant Quarantine Agency, Anyangsi, Gyeonggido 14089, Republic of Korea
2Department of Integrated Biomedical and Life Science, College of Health Science, Korea University, Seoul 02841, Republic of Korea
3Department of Public Health Science (Brain Korea 21 PLUS Program), Graduate School, Korea University, Seoul 02841, Republic of Korea
4Chungcheongbukdo Veterinary Service, Chungjusi, Chungcheongbukdo 380-230, Republic of Korea
Correspondence to: Tel: +82-31-467-1823; Fax: +82-31-467-1778; E-mail: kimjm88@korea.kr (JM Kim), yhlim@korea.ac.kr (YH Lim)
Received: July 11, 2016; Revised: October 11, 2016; Accepted: January 2, 2017; Published online: April 6, 2017.
The aim of this study was to investigate the molecular characteristics and conduct a comparative genomic analysis for Mycobacterium bovis strain 1595 isolated from a native Korean cow. Molecular typing showed that M. bovis 1595 has spoligotype SB0140 with mycobacterial interspersed repetitive units–variable number of tandem repeats typing of 4-2-5-3-2-7-5-5-4-3-4-3-4-3, representing the most common type of M. bovis in Korea. The complete genome sequence of strain 1595 was determined using Illumina MiSeq and PacBio single-molecule real-time technology, which showed a genome of 4,351,712 bp in size with a 65.64% G+C content and 4,358 protein-coding genes. Comparative genomic analysis with the genomes of Mycobacterium tuberculosis complex strains revealed that all genomes are similar in size and G+C content. In the phylogenetic analysis, all strains were within a 0.1% average nucleotide identity value, and MUMmer analysis illustrated that all genomes showed positive collinearity with strain 1595. Sequence comparison based on BLASTP analysis showed that M. bovis AF2122/97 was the strain with the greatest number of completely matched proteins to M. bovis 1595. This genome sequence analysis will serve as a valuable reference for improving understanding of the virulence and epidemiologic traits among M. bovis isolates in Korea.
Keywords: Mycobacterium bovis, South Korea, cattle, Molecular Diagnostic Testing, Genomics

© 2017 The Korean Society of Veterinary Science.