KCHO-1(Mecasin), a novel herbal anti-inflammatory compound, attenuates oxidative stress in an animal model of amyotrophic lateral sclerosis.
Myung Geun Kook1,2, Soon Won Choi1,2, Yoojin Seo1,2, Dong Woung Kim3, Bong Keun Song4, Ilhong Son5 , Sungchul Kim6,*, Kyung-Sun Kang1,2,*
1Adult Stem Cell Research Center, College of Veterinary Medicine, Seoul National University, Seoul, Republic of Korea
2Research Institute for Veterinary Science, College of Veterinary Medicine, Seoul National University, Seoul, Republic of Korea
3Center of Integrative Medicine, Department of Internal Medicine, Wonkwang University Gwangju Hospital, 543-8 JuweolI-Dong, Nam-Gu, Gwangju, 503-310, Republic Korea
4Department of Internal Medicine, School of Oriental Medicine, Wonkwang University, Iksan, Republic of Korea
5Department of Neurology, Inam Neuroscience Research Center, Sanbon Medical Center, College of Medicine, Wonkwang University, Iksan, Republic of Korea
6ALS/MND Center of Wonkwang University Korean Medical Hospital, 543-8 Juwol Dong, Nam-gu, Gwangju 503-310, Republic of Korea
Correspondence to: Tel: +82-2-880-1298; Fax: +82-2-876-7610; E-mails: kangpub@snu.ac.kr (KS Kang), kscndl@hanmail.net (S Kim)
Received: July 14, 2016; Revised: December 27, 2016; Accepted: February 7, 2017; Published online: April 6, 2017.
Abstract
Amyotrophic lateral sclerosis (ALS) is a neurodegenerative disease characterized by selective death of motor neurons in the central nervous system. The main cause of the disease is still elusive but several mutations have been associated with the disease process. In particular, mutant SOD1 protein causes oxidative stress by activating glia cells and contributes to the motor neuron degeneration. KCHO-1, a novel herbal combination compound, contains 30% ethanol and the extracts of nine herbs that have been commonly used in traditional medicine to prevent fatigue or inflammation. In this study, we investigated whether KCHO-1 administration could reduce oxidative stress in an ALS model. KCHO-1 administration to ALS model mice improved the motor function and delayed the disease onset. Furthermore, we found that KCHO-1 administration reduced oxidative stress through gp91phox and MAPK pathway both in classically activated microglia and in the spinal cord of hSOD1G93A transgenic mice. These data suggest that KCHO-1 play as an effective therapeutic agent for ALS by reducing oxidative stress.
Keywords: Amyotrophic lateral sclerosis, Traditional medicine, Neurodegenerative disease, Oxidative stress, gp91phox


© 2017 The Korean Society of Veterinary Science.