Pharmacokinetics of tilmicosin in healthy pigs and in pigs experimentally infected with Haemophilus parasuis
Ling Zhang1,2,3,†, Li Zhao1,2,†, Yonghong Liu1,2, Junfeng Liu2, Xianqiang Li1,2,*
1College of Animal Science and Technology, Tarim University, Alar, Xinjiang 843300, China
2Key Laboratory of Tarim Animal Husbandry Science and Technology of Xinjiang Production & Construction Corps, Alar, Xinjiang 843300, China
3College of Animal Science & Technology, Shihezi University, Shihezi, Xinjiang 832000, China
Correspondence to: Tel: +86-15899339804; Fax: +86-997-4680332; E-mail: lixianqiang89@sina.com
The first two authors contributed equally to this work.
Received: October 21, 2016; Revised: December 10, 2016; Accepted: February 7, 2017; Published online: April 6, 2017.
Abstract
A comparative in vivo pharmacokinetic (PK) study of tilmicosin (TIL) was conducted in 6 crossbred healthy pigs and 6 crossbred pigs infected with Haemophilus parasuis (H. parasuis) following oral administration of a single dose of 40 mg/kg. The infected model was established by intranasal inoculation and confirmed by clinical signs, blood biochemical and microscopic examination. TIL concentrations in plasma were determined by a validated high-performance liquid chromatography (HPLC) method with ultra violet detection at a wavelength of 285 nm and PK parameters were calculated by using the Winnonlin software. After administration, the main PK parameters of TIL in healthy and H. parasuis-infected pigs were as follows: the area under the concentration-time curve (AUC0-t), maximal drug concentration (Cmax), half-life of the absorption phase (t1/2Ka), half-life of the distribution phase (t1/2α) and half-life of the elimination phase (t1/2β) were 34.86±9.69 versus 28.73±6.18 μg•h/mL, 1.77±0.33 versus 1.67±0.28 μg/mL, 2.27±0.45 versus 2.24±0.44 h, 5.35±1.40 versus 4.61±0.35 h, and 43.53±8.17 versus 42.05±9.36 h, respectively. Those parameters suggest that TIL was absorbed quickly and completely, distributed widely, and eliminated slowly in the infected pigs, and there was no statistically significant difference between the PK profiles for the infected and healthy pigs.
Keywords: Tilmicosin; HPLC; Pharmacokinetics; Infected pigs; Haemophilus parasuis


© 2017 The Korean Society of Veterinary Science.