Quantitative evaluation of renal parenchymal perfusion using contrast-enhanced ultrasonography in renal ischemia-reperfusion injury in dogs
Gahyun Lee, Sunghoon Jeon, Sang-kwon Lee, Byunggyu Cheon, Sohyeon Moon, Jun-Gyu Park, Kyoung-Oh Cho, Jihye Choi*
College of Veterinary Medicine, Chonnam National University, Gwangju 61186, Korea
Correspondence to: Tel: +82-62-530-2821; E-mail: imsono@chonnam.ac.kr
Received: October 24, 2016; Revised: January 9, 2017; Accepted: February 7, 2017; Published online: April 6, 2017.
Abstract
This study evaluated whether renal perfusion changes can be noninvasively estimated using contrast-enhanced ultrasonography (CEUS) in renal ischemia-reperfusion injury and investigated the correlation between renal perfusion measured by CEUS and necrosis and apoptosis of renal tubular epithelial cell. In six dogs with experimentally induced renal ischemia-reperfusion injury, changes in time to peak intensity, peak intensity and area under the curve were measured on CEUS. Peak intensity and area under the curve of the renal cortex began to decrease by about 20% compared to baseline from day 1, and reached its lowest level, about 50% to baseline, on day 4. It then gradually increased until day 10; Peak intensity was about 87% and area under the curve was about 95% to the baseline and both were not completely recovered. Both parameters were strongly correlated with the necrosis scores on histopathologic examination on day 4 (r = -0.810 of peak intensity and r = -0.886 of area under the curve). CEUS could quantitatively evaluate perfusion change of acute renal ischemia-reperfusion injury and was correlated with renal tubular damage on histopathologic examination. CEUS could be used as a noninvasive and quantitative diagnostic method for determining the progress of renal ischemia-reperfusion injury.
Keywords: contrast-enhanced ultrasonography, ischemia-reperfusion injury, renal tubular damage, time-intensity curve, dog


© 2017 The Korean Society of Veterinary Science.