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J. Vet. Sci. 2017; 18(2): 119-127  https://doi.org/10.4142/jvs.2017.18.2.119
Neuronal maturation in the hippocampal dentate gyrus via chronic oral administration of Artemisa annua extract is independent of cyclooxygenase 2 signaling pathway in diet-induced obesity mouse model
Hye Kyung Baek1,†, Pan Soo Kim2,†, Ji Ae Song1, Dong-Hwa Choi2, Do Eun Kim1, Seung Il Oh3, Sang-Kyu Park3, Sung-Jo Kim4, Ki-Duk Song5, In Koo Hwang6, Hyung Seok Seo7, Sun Shin Yi1,*
Departments of 1Biomedical Laboratory Science, and 3Medical Biotechnology, College of Medical Sciences, Soonchunhyang University, Asan 31538, Korea
2Biocenter, Gyeonggi Institute of Science and Technology Promotion (GSTEP), Suwon 16229, Korea
4Department of Biotechnology, Hoseo University, Asan 31499, Korea
5Department of Animal Biotechnology, College of Agricultural Life Science, ChonBuk National University, Jeonju 54896, Korea
6Department of Anatomy and Cell Biology, College of Veterinary Medicine, Seoul National University, Seoul 08826, Korea
7Department of Health Science, Konyang University, Nonsan 32992, Korea
Correspondence to: Sun Shin Yi Tel: +82-41-530-4873; Fax: +82-41-530-3085; E-mail: admiral96@sch.ac.kr
†The first two authors contributed equally to this work.
Received: March 17, 2016; Revised: June 21, 2016; Accepted: July 21, 2016; Published online: June 30, 2017.
Abstract
Recently, we reported that Artemisia annua (AA) has anti-adipogenic properties in vitro and in vivo. Reduction of adipogenesis by AA treatment may dampen systemic inflammation and protect neurons from cytokine-induced damage. Therefore, the present study was undertaken to assess whether AA increases neuronal maturation by reducing inflammatory responses, such as those mediated by cyclooxygenase 2 (COX-2). Mice were fed normal chow or a high-fat diet with or without chronic daily oral administration of AA extract (0.2 g/10 mL/kg) for 4 weeks; then, changes in their hippocampal dentate gyri were measured via immunohistochemistry/immunofluorescence staining for bromodexoxyuridine, doublecortin, and neuronal nuclei, markers of neuronal maturation, and quantitative western blotting for COX-2 and Iba-1, in order to assess correlations between systemic inflammation (interleukin-6) and food type. Additionally, we tested the effect of AA in an Alzheimer’s disease model of Caenorhabditis elegans and uncovered a potential benefit. The results show that chronic AA dosing significantly increases neuronal maturation, particularly in the high-fat diet group. This effect was seen in the absence of any changes in COX-2 levels in mice given the same type of food, pointing to the possibility of alternate anti-inflammatory pathways in the stimulation of neurogenesis and neuro-maturation in a background of obesity.
Keywords: Artemisia annua, anti-obesity, cyclooxygenase 2, neurogenesis, neuro-maturation


© 2017 The Korean Society of Veterinary Science.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.