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J. Vet. Sci. 2017; 18(2): 141-148  https://doi.org/10.4142/jvs.2017.18.2.141
Effect of donor age on the proliferation and multipotency of canine adipose-derived mesenchymal stem cells
Jienny Lee, Keum Sil Lee, Chan-Lan Kim, Jeong Su Byeon, Na-Yeon Gu, In-Soo Cho, Sang-Ho Cha*
Viral Disease Research Division, Animal and Plant Quarantine Agency, Gimcheon 39660, Korea
Correspondence to: Sang-Ho Cha Tel: +82-54-912-0805; Fax: +82-54-912-0812; E-mail: virusmania@korea.kr
Received: August 24, 2015; Revised: April 19, 2016; Accepted: June 8, 2016; Published online: June 30, 2017.
Abstract
Research into adipose tissue-derived mesenchymal stem cells (AD-MSCs) has demonstrated the feasibility of their use in clinical applications due to their ease of isolation and abundance in adipose tissue. We isolated AD-MSCs from young and old dogs, and the cells were subjected to sequential sub-passaging from passage 1 (P1) to P7. Canine AD-MSCs (cAD-MSCs) were examined for proliferation kinetics, expression of molecules associated with self-renewal, expression of cell surface markers, and differentiation potentials at P3. Cumulative population doubling level was significantly higher in cAD-MSCs of young donors than in those of old donors. In addition, expressions of CD73, CD80, Oct3/4, Nanog, cell survival genes and differentiation potentials were significantly higher in young donors than in old donors. The present study suggests that donor age should be considered when developing cell-based therapies for clinical application of cAD-MSCs.
Keywords: adipose mesenchymal stem cells, age, canine, differentiation, multipotency


© 2017 The Korean Society of Veterinary Science.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.