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J. Vet. Sci. 2017; 18(2): 149-158  https://doi.org/10.4142/jvs.2017.18.2.149
Estrogen receptor-α, progesterone receptor, and c-erbB/HER-family receptor mRNA detection and phenotype analysis in spontaneous canine models of breast cancer
Farruk M. Lutful Kabir1,†, Patricia DeInnocentes1, Payal Agarwal2, Christopher P. Mill3,‡, David J. Riese 2nd3, R. Curtis Bird1,*
Auburn University Research Initiative in Cancer (AURIC), 1Department of Pathobiology, 2Scott-Ritchey Research Center, College of Veterinary Medicine, and 3Department of Drug Discovery and Development, Harrison School of Pharmacy, Auburn University, AL 36849, USA
Correspondence to: R. Curtis Bird Tel: +1-334-844-2707; Fax: +1-334-844-2652; E-mail: birdric@auburn.edu
Received: February 11, 2016; Revised: June 2, 2016; Accepted: July 21, 2016; Published online: June 30, 2017.
Abstract
Well characterized, stable, p16-defective canine mammary cancer (CMT) cell lines and normal canine mammary epithelial cells were used to investigate expression of the major breast cancer-specific hormone receptors estrogen receptor alpha (ER1) and progesterone receptor (PR) as well as luminal epithelial-specific proto-oncogenes encoding c-erbB-1 (epidermal growth factor receptor/EGFr), c-erbB-2/HER2, c-erbB-3, and c-erbB-4 receptors. The investigation developed and validated quantitative reverse transcriptase polymerase chain reaction assays for each transcript to provide rapid assessment of breast cancer phenotypes for canine cancers, based on ER1, PR, and c-erbB-2/HER2 expressions, similar to those in human disease. Roles for relatively underexplored c-erbB-3 and c-erbB-4 receptor expressions in each of these breast cancer phenotypes were also evaluated. Each quantitative assay was validated by assessment of amplicon size and DNA sequencing following amplification. Differential expression of ER1, PR, and c-erbB-2 in CMT cell lines clearly defined distinct human-like breast cancer phenotypes for a selection of CMT-derived cell lines. Expression profiles for EGFr family genes c-erbB-3 and c-erbB-4 in CMT models also provided an enriched classification of canine breast cancer identifying new extended phenotypes beyond the conventional luminal-basal characterization used in human breast cancer.
Keywords: breast cancer, canine, mammary epithelial cells, estrogen/progesterone receptor, c-erbB/HER-2 receptor


© 2017 The Korean Society of Veterinary Science.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.