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J. Vet. Sci. 2017; 18(2): 159-167  https://doi.org/10.4142/jvs.2017.18.2.159
Immunogenicity of recombinant Lactobacillus plantarum NC8 expressing goose parvovirus VP2 gene in BALB/c mice
Yu-Ying Liu1, Wen-Tao Yang1, Shao-Hua Shi1, Ya-Jie Li1, Liang Zhao1, Chun-Wei Shi1, Fang-Yu Zhou1, Yan-Long Jiang1, Jing-Tao Hu1, Wei Gu1,2, Gui-Lian Yang1,*, Chun-Feng Wang1,*
1College of Animal Science and Technology, Jilin Provincial Engineering Research Center of Animal Probiotics, Jilin Agricultural University, Changchun 130118, China
2Shandong Baolai-leelai Bioengineering Co. Ltd, Taian 271000, China
Correspondence to: Gui-Lian Yang, Chun-Feng Wang Tel/Fax: +86-43184533425; E-mails: yangguilian@jlau.edu.cn (GL Yang), wangchunfeng@jlau.edu.cn (CF Wang)
Received: September 25, 2015; Revised: April 11, 2016; Accepted: June 8, 2016; Published online: June 30, 2017.
Abstract
Goose parvovirus (GPV) continues to be a threat to goose farms and has significant economic effects on the production of geese. Current commercially available vaccines only rarely prevent GPV infection. In our study, Lactobacillus (L.) plantarum NC8 was selected as a vector to express the VP2 gene of GPV, and recombinant L. plantarum pSIP409-VP2/NC8 was successfully constructed. The molecular weight of the expressed recombinant protein was approximately 70 kDa. Mice were immunized with a 2 × 109 colony-forming unit/200 μL dose of the recombinant L. plantarum strain, and the ratios and numbers of CD11c+, CD3+CD4+, CD3+CD8+, and interferon gamma- and tumor necrosis factor alpha-expressing spleen lymphocytes in the pSIP409-VP2/NC8 group were higher than those in the control groups. In addition, we assessed the capacity of L. plantarum SIP409-VP2/NC8 to induce secretory IgA production. We conclude that administered pSIP409-VP2/NC8 leads to relatively extensive cellular responses. This study provides information on GPV infection and offers a clear framework of options available for GPV control strategies.
Keywords: Lactobacillus plantarum, VP2 gene, goose parvovirus, immunization


© 2017 The Korean Society of Veterinary Science.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.