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J. Vet. Sci. 2017; 18(2): 193-200  https://doi.org/10.4142/jvs.2017.18.2.193
Alterations in antioxidant function and cell apoptosis in duck spleen exposed to molybdenum and/or cadmium
Mengmeng Zhang, Junrong Luo, Caiying Zhang*, Huabin Cao*, Bing Xia, Guoliang Hu
Institute of Animal Population Health, College of Animal Science and Technology, Jiangxi Agriculture University, Nanchang 330045, China
Correspondence to: Caiying Zhang, Huabin Cao Tel: +86-791-83813503; Fax: +86-791-83813504; E-mails: zhangcaiying0916@163.com (C Zhang), chbin20020804@163.com (H Cao)
Received: October 14, 2015; Revised: March 23, 2016; Accepted: May 12, 2016; Published online: June 30, 2017.
Abstract
To investigate the effects of molybdenum (Mo) and/or cadmium (Cd) on antioxidant function and the apoptosis-related genes in duck spleens. Sixty healthy 11-day-old ducks were randomly divided into six groups of 10 ducks (control, low Mo group, high Mo, Cd, low Mo + Cd, and high Mo + Cd groups). All were fed a basal diet containing low or high dietary doses of Mo and/or Cd. Relative spleen weight, antioxidant indices, apoptosis-related gene mRNA expression levels, and ultrastructural changes were evaluated after 120 days. The results showed that the relative spleen weight decreased significantly in the high Mo + Cd treatment group which compared with control group. Malondialdehyde levels increased and xanthine oxidase and catalase activities decreased in the Mo and/or Cd groups compared with levels in the control group. Bak-1 and Caspase-3 expressions were upregulated in the high Mo + Cd group, while Bcl-2 was downregulated. In addition, mitochondrial crest fracture, swelling, vacuolation, deformed nuclei, and karyopyknosis in both Mo + Cd treated groups were more severe than in the other groups. The results suggest that Mo and/or Cd can induce oxidative stress and apoptosis of spleen via effects on the mitochondrial intrinsic pathway. Moreover, the results indicate the two elements have a possible synergistic relationship.
Keywords: apoptosis, cadmium, duck, molybdenum, oxidative stress


© 2017 The Korean Society of Veterinary Science.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.