• home
  • articles
  • authors
  • Reviewers
  • About the Journal
  • About the Journal
  • About the Journal
  • About the Journal
  • About the Journal
  • e-Submission

Indexed/Covered by

J. Vet. Sci. 2017; 18(2): 217-227  https://doi.org/10.4142/jvs.2017.18.2.217
Bovine adenovirus type 3 virions cannot be rescued in vivo after full-length viral genome transfection in the absence of detectable polypeptide IX
Peng Zhang1,†, Qinghong Xue2,†, Jing Ma1, Jingjing Ren1, Shuili Xia1, Lu Zhang1, Wenbin Wang1, Suresh K. Tikoo3,4,*, Enqi Du1,*
1College of Veterinary Medicine, North-west A&F University, Yangling 712100, China
2Chinese Institute of Veterinary Drug Controls, Beijing 100000, China
3VIDO-InteVac, and 4Vaccinology & Immunotherapeutics Program, School of Public Health, University of Saskatchewan Saskatoon, Saskatchewan S4S 6A6, Canada
Correspondence to: Suresh K. Tikoo, Enqi Du Tel: +86-29-87091106; Fax: +86-29-87091032; E-mails: duenqi227@126.com (E Du), suresh.tik@usask.ca (SK Tikoo)
Received: November 18, 2015; Revised: May 30, 2016; Accepted: August 26, 2016; Published online: June 30, 2017.
Abstract
Bovine adenovirus type 3 (BAdV3) is being used in the development of potential vehicles for gene therapy and vectored vaccine. To that end, a more comprehensive description of BAdV3 biology is essential. In this study, we focused on the role of pIX in BAdV3 virion rescue after full-length BAdV3 genome transfection. Initially, pIX deletion or initiation codon mutation abolished the production of progeny virions, which suggested that pIX was essential for the rescue of BAdV3 containing a full-length genome. Moreover, through transfection of a panel of pIX mutant BAdV3 genomes, we observed that the conserved N-terminus and the putative leucine zipper element (PLZP) were essential for virion rescue, whereas the C-terminus following the coiled-coil domain was non-essential. In addition, swap of the PLZP element and its following region of BAdV3 pIX to corresponding domains of human adenovirus type 5 (HAdV5) did not affect virion production, whereas swap of the entire pIX abolished production of progeny virions. We suggest that failure of the full-length BAdV3 pIX swap might be due to species specificity of its N-terminus region before the PLZP element.
Keywords: adenovirus, bovine adenovirus type 3, polypeptide IX, viral rescue


© 2017 The Korean Society of Veterinary Science.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.