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J. Vet. Sci. 2017; 18(2): 237-243  https://doi.org/10.4142/jvs.2017.18.2.237
Sequence analysis of the spike gene of Porcine epidemic diarrhea virus isolated from South China during 2011-2015
Xiaoya Zhao1, Zhili Li2, Xiduo Zeng3, Guanqun Zhang2,3,†, Jianqiang Niu2,3,†, Baoli Sun1, Jingyun Ma1,*
1College of Animal Science, South China Agricultural University, Guangzhou 510642, China
2Henan University of Science and Technology, Luoyang 471900, China
3Guangdong Wen’s Food Co., Ltd., Xinxing 527400, China
Correspondence to: Jingyun Ma Tel: +86-20-8528-0283; Fax: +86-20-8528-0283; E-mail: majy2400@scau.edu.cn
Received: December 28, 2015; Revised: June 25, 2016; Accepted: July 21, 2016; Published online: June 30, 2017.
Abstract
The spike gene of porcine epidemic diarrhea virus (PEDV) was sequenced from 55 South China field strains isolated from pigs with symptoms of diarrhea. The sequences were compared within the set of field strains as well as with reference strains available in GenBank. Within the 55 South China PEDV field strains, the deduced amino acid sequence identities ranged from 93.8% to 99.9 % and ranged from 90.7% to 99.5% when compared with the foreign reference strains in GenBank. Our phylogenetic analysis showed that 10 of the 55 South China PEDV strains belonged to G1b and 45 belonged to G2b.
Keywords: Porcine epidemic diarrhea virus, molecular characterization, phylogenetic analysis


© 2017 The Korean Society of Veterinary Science.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.