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J. Vet. Sci. 2017; 18(2): 245-251  https://doi.org/10.4142/jvs.2017.18.2.245
Efficacy of horse chestnut leaf extract ALH-L1005 as a matrix metalloproteinase inhibitor in ligature-induced periodontitis in canine model
Se Eun Kim1, Tae Hyun Kim1, Shin Ae Park1, Won Tae Kim1, Young Woo Park1, Jae Sang Ahn1, Manbok Jeong1, Min-Young Kim2, Kangmoon Seo1,*
1Department of Veterinary Surgery, College of Veterinary Medicine, Seoul National University, Seoul 08826, Korea
2AngioLab, Inc. Daejeon 34016, Korea
Correspondence to: Kangmoon Seo Tel: +82-2-880-1258; Fax: +82-2-884-8651; E-mail: kmseo@snu.ac.kr
Received: February 11, 2016; Revised: April 28, 2016; Accepted: July 21, 2016; Published online: June 30, 2017.
Matrix metalloproteinases (MMPs) are the main proteinases associated with periodontal tissue destruction and remodeling. Therefore, inhibition of host-derived MMPs has a key role in the prevention and reduction of periodontitis progression. Horse chestnut (Aesculus hippocastanum L.) extracts have been used as treatments for inflammatory disease, traditionally. This study assessed the clinical effect as a MMP inhibitor of horse chestnut leaf extract ALH-L1005 on periodontitis. ALH-L1005 was obtained from horse chestnut leaf and its MMP inhibitory activities estimated. Periodontitis was induced in beagles assigned to 4 groups and medicated for 6 weeks: low dose test (LT; ALH-L1005, 100 mg/kg/day), high dose test (HT; ALH-L1005, 200 mg/kg/day), positive control (PC; doxycycline, 10 mg/kg/day), or negative control (NC; placebo). Before and after administration, clinical indices of the teeth and MMP quantity in gingival tissues using zymography were measured. Clinical conditions of the LT, HT, and PC groups were significantly improved after 6 weeks. In zymographic evaluations, gelatinolytic and caseinolytic activities were suppressed in LT, HT, and PC groups but not in the NC group. The results suggest that ALH-L1005 could be an effective agent for clinical prevention and treatment of periodontitis by inhibiting the gelatinase and collagenase activities, which can detach periodontal ligaments from alveolar bone.
Keywords: dog, doxycycline, horse chestnut leaf extract, matrix metalloproteinase, periodontal disease

© 2017 The Korean Society of Veterinary Science.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.