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Chicken RNA-binding protein TIA-1 contributes to stress granule formation in chicken cells and tissues
Yingjie Sun1, Pin Zhang2, Hang Zheng3, Luna Dong3, Lei Tan1, Cuiping Song1, Xusheng Qiu1, Ying Liao1, Chunchun Meng1, Shengqing Yu1, Chan Ding1,4,*
1Department of Avian Infectious Diseases, Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Science, Shanghai 200241, China
2College of Animal Science and Technology, Shandong Agricultural University, Taian 271018, China
3College of Animal Science and Technology, Jilin Agricultural University, Changchun 130118, China
4Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou 225009, China
Correspondence to: Tel: +86-21-34293441; Fax: +86-21-54081818; E-mail: shoveldeen@shvri.ac.cn
Received: September 27, 2016; Revised: February 10, 2017; Accepted: March 26, 2017; Published online: July 10, 2017.
Abstract
T-cell internal antigen-1 (TIA-1) plays roles in regulating alternative pre-mRNA splicing, mRNA translation, and stress granule (SG) formation in human cells. As an evolutionarily conserved response to environmental stress, SG has been reported to exist in various species. However, SG formation in chicken cells and the role of chicken TIA-1 (cTIA-1) in SG assembly has not been elucidated. In the present study, we cloned cTIA-1 and showed that cTIA-1 facilitates in the assembly of canonical SGs in both human and chicken cells. Overexpression of chicken prion-related domain (cPRD) of cTIA-1 that bore an N-terminal GFP tag (pntGFP-cPRD) or Flag tag (pFlag-cPRD) induced the production of typical SGs. However, C-terminal GFP tag (pctGFP-cPRD) induced significantly large cytoplasmic granules that were devoid of endogenous G3BP1 and remained stable when exposed to cycloheximide, indicating that these were not typical SGs, and pntGFP tag influences cPRD localization. Finally, endogenous cTIA-1 was determined to be recruited to SGs in chicken cells and tissues under environmental stress. Taken together, our studies provide evidence that chicken TIA-1 plays a role in canonical SG formation in chicken cells and tissues. Our findings also indicated that cPRD is necessary for SG aggregation.
Keywords: TIA-1, chicken, prion-related domain, stress granule, tissues


© 2017 The Korean Society of Veterinary Science.