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Investigation of bovine tuberculosis outbreaks using the trace-back system and molecular typing in Korean Hanwoo beef cattle
Bok Kyung Ku1,*,†, Bo-Young Jeon2,*,†, Jae Myung Kim1, Young-Boo Jang1, Hyeyoung Lee2, Jae Young Choi1, Suk Chan Jung1, Hyang-Mi Nam3, Hun Park4, Sang-Nae Cho5
1Bacterial Disease, Department of Animal and Plant Health Research, Animal and Plant Quarantine Agency, Gimcheon 39660, Korea
2Department of Biomedical Laboratory Science, College of Health Science, Yonsei University, Wonju 26493 Korea
3Foreign Animal Disease, Department of Animal and Plant Health Research, Animal and Plant Quarantine Agency, Gimcheon 39660, Korea
4Namwon Branch, Jeonbuk Livestock & Veterinary Service, Namwon 55725, Koerea
5Department of Microbiology, College of Medicine, Yonsei University, Seoul 03722 Korea
Correspondence to: Tel: +82-33-760-5108; Fax: +82-504-841-5108; E-mails: kubk@korea.kr (BK Ku), bojeon@yonsei.ac.kr (BY Jeon)
The first two authors contributed equally to this work.
Received: November 8, 2016; Revised: February 13, 2017; Accepted: April 13, 2017; Published online: July 10, 2017.
Abstract
Bovine tuberculosis is a chronic contagious disease responsible for major agricultural economic losses. Abattoir monitoring and trace-back systems are an appropriate method to control bovine tuberculosis, particularly in beef cattle. In the present study, a trace-back system was applied to bovine tuberculosis outbreaks of Korean native Hanwoo beef cattle. Bovine tuberculosis was detected in three index beef cattle during abattoir monitoring in Jeonbuk Province, Korea, and the original herds were traced back from each index cattle. All cattle in the original herds were subjected to tuberculin skin test. The positive rates of the tuberculin skin test were 64.2% (62 of 96), 2.4% (2 of 42) and 8.1% (3 of 37) at farms A, B and C, respectively. In the post-mortem examination of 56 tuberculin-positive cattle, 62% had granulomatous lesions, and Mycobacterium bovis was cultured from 40 (71.4%) cattle. Molecular typing by spoligotyping and the mycobacterial interspersed repetitive unit-variable number tandem repeat assay revealed the genotype of the M. bovis strains from the index cattle were same as the M. bovis genotype in each original herd. These results suggest that tracing back from the index cattle to the original herd is an effective method to control bovine tuberculosis in beef cattle.
Keywords: Korean beef cattle (Hanwoo), bovine tuberculosis, index beef cattle, molecular typing, track-back system


© 2017 The Korean Society of Veterinary Science.